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XB-GRNA-23659353
gRNA Name:
dmrt1 gRNA2
Synonyms: dmrt1.L
Genomic Target: dmrt1
PAM Sequence: CGG
Sequence: 5' CCCGTAACTCCGGGCAGCACCGG 3'
Synonyms: dmrt1.L
Genomic Target: dmrt1
PAM Sequence: CGG
Sequence: 5' CCCGTAACTCCGGGCAGCACCGG 3'
Genomic Target | laevis.L | laevis.S | |||||
Identities | 21/23 | 19/23 | |||||
genome
gRNA
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5' TGGGTGCTGCCCGGAGTTACGGG 3'
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3' GGCCACGACGGGCCTCAATGCCC 5' |
5' TGGGTGCTGCCCGGTGCTACGGG 3' |||||||||||| | |||||| 3' GGCCACGACGGGCCTCAATGCCC 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 10.1 | Chr1L:139345608..139345630 | 21/23 | Antisense | target | dmrt1.L | ||
Chr1S:118842681..118842703 | 19/23 | Antisense | target | dmrt1.S | tropicalis 10.0 | Chr3:152285098..152285120 | 19/23 | Antisense | off-target | tfr2 |
Chr1:59608796..59608818 | 20/23 | Sense | unknown | ||||
Publications | |||||||
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