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Summary Attributions Wiki
XB-GRNA-23659199

gRNA Name: rbl1 gRNA2
Synonyms: rbl1{cr1}
Genomic Target: rbl1

PAM Sequence: AGG
Sequence: 5' ATATTTCAAAACCCTCACG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/19 18/19 18/19
genome
gRNA
5' CGTGAGGGTTTTGAAATAT 3'
   |||||||||||||||||||
3' GCACTCCCAAAACTTTATA 5'
5' CGTGAGGATTTTGAAATAT 3'
   ||||||| |||||||||||
3' GCACTCCCAAAACTTTATA 5'
5' CGTGGGGGTTTTGAAATAT 3'
   |||| ||||||||||||||
3' GCACTCCCAAAACTTTATA 5'
   
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr9_10L:34256002..34256020 18/19 Antisense target rbl1.L
chr9_10S:30889849..30889867 18/19 Antisense target rbl1.S
chr8L:10200671..10200689 16/19 Sense off-target nr6a1.L
chr3L:90823924..90823942 17/19 Antisense unknown
tropicalis 10.0 Chr10:42364406..42364424 19/19 Sense target rbl1
Chr2:127558305..127558323 17/19 Sense off-target dach1
Chr1:105615341..105615359 17/19 Sense off-target madcam1
Chr8:87437086..87437104 16/19 Sense off-target slc24a4
Chr6:4611127..4611145 16/19 Sense off-target LOC101734654
Chr6:4611127..4611145 16/19 Sense off-target LOC101734591
Chr6:4627293..4627311 16/19 Sense off-target LOC101733242
Chr6:4627293..4627311 16/19 Sense off-target LOC101734654
Chr6:4637879..4637897 16/19 Sense off-target LOC101733242
Chr6:4637879..4637897 16/19 Sense off-target LOC101734654
Chr6:4651450..4651468 16/19 Sense off-target LOC101733242
Chr6:4651450..4651468 16/19 Sense off-target LOC101734654
Chr7:90533409..90533427 17/19 Sense unknown
Chr1:191676405..191676423 16/19 Sense unknown
Chr4:125083620..125083638 16/19 Antisense unknown
Chr7:126979716..126979734 16/19 Sense unknown
Publications
First: CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis., Sci Rep 2016                            
Most recent:
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