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Summary Attributions Wiki
XB-GRNA-23659198
gRNA Name: rb1 gRNA3
Synonyms: rb1{cr2}
Genomic Target: rb1

PAM Sequence: CGG
Sequence: 5' GCTGTATGATTGTGCTGTAC 3'



Genomic Target tropicalis laevis.S
Identities 20/20 20/20
genome
gRNA
 
5' GTACAGCACAATCATACAGC 3'
   ||||||||||||||||||||
3' CATGTCGTGTTAGTATGTCG 5'
 
5' GTACAGCACAATCATACAGC 3'
   ||||||||||||||||||||
3' CATGTCGTGTTAGTATGTCG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2S:126520774..126520793 20/20 Antisense target rb1.S
Chr3S:61298843..61298862 19/20 Sense off-target LOC108712994
Chr9_10S:60721462..60721481 18/20 Antisense off-target kcnj3.S
Chr1L:105367468..105367487 18/20 Sense off-target LOC108712234
Chr1L:105367468..105367487 18/20 Sense off-target LOC108712235
Chr1S:136171824..136171843 18/20 Sense off-target LOC108706439
Chr2L:141521817..141521836 19/20 Antisense unknown
Chr8S:18223292..18223311 18/20 Antisense unknown
Chr4S:79070080..79070099 17/20 Sense unknown
Chr5S:59279752..59279771 17/20 Antisense unknown
Chr7L:68253800..68253819 17/20 Sense unknown
tropicalis 10.0 Chr2:135006672..135006691 20/20 Antisense target rb1
Chr3:38492205..38492224 18/20 Sense off-target nr3c1
Chr7:55940101..55940120 17/20 Antisense off-target clstn1
Chr3:47962519..47962538 16/20 Antisense off-target gfra3
Chr2:156830855..156830874 16/20 Antisense unknown
Chr8:97249704..97249723 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis., Sci Rep 2016     
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