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Summary Attributions Wiki
XB-GRNA-23659167

gRNA Name: fxr1 gRNA6
Synonyms: T8L fxr1
Genomic Target: fxr1

PAM Sequence: TGG
Sequence: 5' TCAGGACAATGGTTCTTAAATGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 22/23 23/23 20/23
genome
gRNA
 
5' CCATTTAAGAACCATTGTCTTGA 3'
   ||||||||||||||||||| |||
3' GGTAAATTCTTGGTAACAGGACT 5'
 
5' CCATTTAAGAACCATTGTCCTGA 3'
   |||||||||||||||||||||||
3' GGTAAATTCTTGGTAACAGGACT 5'
 
5' CCATTTCAGAACCATTGTCTTTA 3'
   |||||| |||||||||||| | |
3' GGTAAATTCTTGGTAACAGGACT 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr5L:104553585..104553607 23/23 Antisense target fxr1.L
chr5S:89185604..89185626 20/23 Antisense target fxr1.S
chr1L:179466899..179466921 21/23 Sense off-target ttc37.L
chr8S:29794972..29794994 21/23 Sense off-target col5a1.S
chr1S:68393776..68393798 20/23 Sense off-target btc.S
chr6L:71408457..71408479 20/23 Sense off-target LOC108719059
chr1L:1915595..1915617 19/23 Antisense off-target ctnna2.L
chr2S:119944122..119944144 19/23 Sense off-target ankrd52.S
chr1S:7681386..7681408 19/23 Sense off-target LOC108706501 [provisional:ctnna2]
chr5L:75716866..75716888 19/23 Sense off-target LOC108716160 [provisional:gprc6a]
chr5L:9037884..9037906 20/23 Sense unknown
chr7L:123399723..123399745 20/23 Antisense unknown
chr8S:6528201..6528223 20/23 Sense unknown
Scaffold20389:353..375 19/23 Sense unknown
chr1S:86607458..86607480 19/23 Antisense unknown
chr2S:139673468..139673490 19/23 Antisense unknown
chr5L:37754164..37754186 19/23 Sense unknown
chr7S:30217917..30217939 19/23 Antisense unknown
tropicalis 10.0 Chr5:108318945..108318967 22/23 Antisense target fxr1
Chr1:58511030..58511052 20/23 Antisense off-target znf827
Chr9:49725024..49725046 19/23 Antisense off-target ccnyl1
Chr1:127000230..127000252 20/23 Sense unknown
Chr6:114240556..114240578 19/23 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells., J Cell Biol 2020                            
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