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Summary Attributions Wiki
XB-GRNA-23659165
gRNA Name: fxr1 gRNA4
Synonyms: T7L fxr1
Genomic Target: fxr1

PAM Sequence: TGG
Sequence: 5' ACTGCAGGTTGCAAACATATTGG 3'



Genomic Target laevis.L
Identities 23/23
genome
gRNA
 
5' CCAATATGTTTGCAACCTGCAGT 3'
   |||||||||||||||||||||||
3' GGTTATACAAACGTTGGACGTCA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr5L:104553928..104553950 23/23 Antisense target fxr1.L
chr6L:62653791..62653813 21/23 Sense off-target eepd1.L
chr6L:108275742..108275764 20/23 Sense off-target rock1.L
chr7L:85399598..85399620 20/23 Sense off-target prkcz.L
chr9_10L:74186369..74186391 20/23 Sense off-target LOC108701425
chr9_10L:74186369..74186391 20/23 Sense off-target LOC108701857 [provisional:spag16]
chr9_10S:53242556..53242578 19/23 Antisense off-target neb.S
chr3L:94984376..94984398 19/23 Sense off-target trpm7.L
chr8L:17529426..17529448 19/23 Antisense off-target pappa.L
chr8L:17532145..17532167 19/23 Antisense off-target pappa.L
chr4L:240075..240097 19/23 Sense off-target tsg101.L
chr9_10L:78988554..78988576 19/23 Antisense off-target nbeal1.L
Scaffold22:5999930..5999952 19/23 Sense off-target Xelaev18001643m
chr3L:126558025..126558047 19/23 Antisense off-target Xelaev18018911m
Scaffold42:1244414..1244436 19/23 Sense off-target LOC108704650 [provisional:tyr]
chr7S:6809456..6809478 19/23 Antisense off-target LOC108697174 [provisional:pcdh15]
chr2S:98120903..98120925 21/23 Antisense unknown
chr9_10L:25924235..25924257 21/23 Antisense unknown
chr2L:107435853..107435875 20/23 Sense unknown
chr6L:44528056..44528078 20/23 Antisense unknown
Scaffold97570:11..33 19/23 Antisense unknown
chr1S:113323034..113323056 19/23 Antisense unknown
chr3L:126510941..126510963 19/23 Antisense unknown
chr8S:78195134..78195156 19/23 Sense unknown
tropicalis 10.0 Chr6:36256846..36256868 19/23 Sense off-target etv1
Chr1:184943894..184943916 19/23 Antisense off-target crhbp
Chr3:104816036..104816058 19/23 Sense off-target trpm7
Chr6:94613256..94613278 19/23 Sense off-target cdkal1
Chr1:121123987..121124009 19/23 Sense unknown
Chr6:116815119..116815141 19/23 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells., J Cell Biol 2020     
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