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XB-GRNA-23659164
gRNA Name:
fxr1 gRNA3
Synonyms: T3 fxr1
Genomic Target: fxr1
PAM Sequence: AGG
Sequence: 5' GGCAGAAGATAGACAGCCAGG 3'
Synonyms: T3 fxr1
Genomic Target: fxr1
PAM Sequence: AGG
Sequence: 5' GGCAGAAGATAGACAGCCAGG 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 21/21 | 21/21 | 21/21 | ||||
genome
gRNA
|
5' CCTGGCTGTCTATCTTCTGCC 3' ||||||||||||||||||||| 3' GGACCGACAGATAGAAGACGG 5' |
5' CCTGGCTGTCTATCTTCTGCC 3' ||||||||||||||||||||| 3' GGACCGACAGATAGAAGACGG 5' |
5' CCTGGCTGTCTATCTTCTGCC 3' ||||||||||||||||||||| 3' GGACCGACAGATAGAAGACGG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr5L:104553704..104553724 | 21/21 | Antisense | target | fxr1.L | ||
chr5S:89185737..89185757 | 21/21 | Antisense | target | fxr1.S | |||
chr6S:60723088..60723108 | 19/21 | Antisense | off-target | basp1.S | |||
chr3L:86204260..86204280 | 19/21 | Antisense | off-target | Xelaev18018125m | |||
chr7L:3554918..3554938 | 18/21 | Sense | off-target | LOC108696016 [provisional:gfra1] | |||
chr9_10L:52059367..52059387 | 17/21 | Antisense | off-target | abcb6.L | |||
chr1L:157946180..157946200 | 17/21 | Sense | off-target | LOC108716320 [provisional:adgrd1] | |||
chr3L:112265252..112265272 | 18/21 | Antisense | unknown | ||||
chr4L:59776676..59776696 | 18/21 | Sense | unknown | ||||
Scaffold229:3524..3544 | 17/21 | Sense | unknown | ||||
chr6L:136500025..136500045 | 17/21 | Antisense | unknown | tropicalis 10.0 | Chr5:108319080..108319100 | 21/21 | Antisense | target | fxr1 |
Chr2:76655751..76655771 | 20/21 | Antisense | unknown | ||||
Chr7:29404622..29404642 | 18/21 | Sense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells., J Cell Biol 2020 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |