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XB-GRNA-23659156
gRNA Name:
prickle1 gRNA1
Synonyms: prickle_L
Genomic Target: prickle1
PAM Sequence: AGG
Sequence: 5' TGCGGAGAGAAGATTAATGG 3'
Synonyms: prickle_L
Genomic Target: prickle1
PAM Sequence: AGG
Sequence: 5' TGCGGAGAGAAGATTAATGG 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 20/20 | 20/20 | 20/20 | ||||
genome
gRNA
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5' CCATTAATCTTCTCTCCGCA 3' |||||||||||||||||||| 3' GGTAATTAGAAGAGAGGCGT 5' |
5' CCATTAATCTTCTCTCCGCA 3' |||||||||||||||||||| 3' GGTAATTAGAAGAGAGGCGT 5' |
5' CCATTAATCTTCTCTCCGCA 3' |||||||||||||||||||| 3' GGTAATTAGAAGAGAGGCGT 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr3L:56107866..56107885 | 20/20 | Sense | target | prickle1.L | ||
chr3S:82057807..82057826 | 20/20 | Antisense | target | prickle1.S | |||
chr5L:16614957..16614976 | 18/20 | Sense | off-target | wdr26.L | |||
chr1S:74604089..74604108 | 18/20 | Antisense | off-target | adgrl3.S | |||
chr1L:168915746..168915765 | 18/20 | Antisense | off-target | slc12a2.L | |||
chr1S:74604089..74604108 | 18/20 | Antisense | off-target | Xelaev18009583m | |||
chr5S:12672231..12672250 | 17/20 | Antisense | off-target | wdr26.S | |||
chr8S:40916324..40916343 | 16/20 | Sense | off-target | psmb7.S | |||
chr3L:107481401..107481420 | 19/20 | Sense | unknown | ||||
chr3S:69765759..69765778 | 17/20 | Sense | unknown | tropicalis 10.0 | Chr3:64772903..64772922 | 20/20 | Sense | target | prickle1 |
Chr4:119570355..119570374 | 18/20 | Sense | off-target | pla2g4a | |||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |