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Summary Attributions Wiki
XB-GRNA-23659147
gRNA Name: sox2 gRNA1
Synonyms:
Genomic Target: sox2

PAM Sequence: AGG
Sequence: 5' TGGTATGGTCCAGGGGCCAG 3'



Genomic Target tropicalis laevis.L
Identities 20/20 20/20
genome
gRNA
 
5' CTGGCCCCTGGACCATACCA 3'
   ||||||||||||||||||||
3' GACCGGGGACCTGGTATGGT 5'
 
5' CTGGCCCCTGGACCATACCA 3'
   ||||||||||||||||||||
3' GACCGGGGACCTGGTATGGT 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 Scaffold33547:574..593 20/20 Antisense target sox2.L
chr2L:115362207..115362226 19/20 Antisense off-target sox1.L
chr3L:39088585..39088604 17/20 Antisense unknown
tropicalis 10.0 Chr5:108037294..108037313 20/20 Antisense target sox2
Chr3:51927364..51927383 16/20 Antisense off-target avpr1a
Chr3:109502565..109502584 16/20 Sense off-target LOC116409779
Chr4:147508789..147508808 19/20 Sense unknown
Chr7:37741897..37741916 17/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
???morpholino.displaySummary.papersRecent??? Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022     
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