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XB-GRNA-23659146
gRNA Name:
smad6 gRNA2
Synonyms: smad6_g2
Genomic Target: smad6
PAM Sequence: AGG
Sequence: 5' GCGGCAGGCAGGTGCATCTA 3'
Synonyms: smad6_g2
Genomic Target: smad6
PAM Sequence: AGG
Sequence: 5' GCGGCAGGCAGGTGCATCTA 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 20/20 | 20/20 | 20/20 | ||||
genome
gRNA
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5' TAGATGCACCTGCCTGCCGC 3' |||||||||||||||||||| 3' ATCTACGTGGACGGACGGCG 5' |
5' TAGATGCACCTGCCTGCCGC 3' |||||||||||||||||||| 3' ATCTACGTGGACGGACGGCG 5' |
5' TAGATGCACCTGCCTGCCGC 3' |||||||||||||||||||| 3' ATCTACGTGGACGGACGGCG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr3L:108458434..108458453 | 20/20 | Antisense | target | smad6.L | ||
chr3S:35721497..35721516 | 20/20 | Sense | target | smad6.S | |||
chr8L:113265171..113265190 | 18/20 | Antisense | off-target | pea15.L | |||
chr1L:75637238..75637257 | 17/20 | Antisense | off-target | slc4a4.L | |||
chr4L:128690069..128690088 | 16/20 | Antisense | off-target | plxna1.L | |||
chr4S:47240760..47240779 | 18/20 | Antisense | unknown | ||||
chr2S:49492619..49492638 | 16/20 | Antisense | unknown | ||||
chr7S:45567541..45567560 | 16/20 | Antisense | unknown | ||||
chr9_10S:43297972..43297991 | 16/20 | Sense | unknown | tropicalis 10.0 | Chr3:117294662..117294681 | 20/20 | Antisense | target | smad6 |
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 |
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???morpholino.displaySummary.papersRecent??? |
Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022 |
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???morpholino.displaySummary.papersAll??? |