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Summary Attributions Wiki
XB-GRNA-23659146
gRNA Name: smad6 gRNA2
Synonyms: smad6_g2
Genomic Target: smad6

PAM Sequence: AGG
Sequence: 5' GCGGCAGGCAGGTGCATCTA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
 
5' TAGATGCACCTGCCTGCCGC 3'
   ||||||||||||||||||||
3' ATCTACGTGGACGGACGGCG 5'
 
5' TAGATGCACCTGCCTGCCGC 3'
   ||||||||||||||||||||
3' ATCTACGTGGACGGACGGCG 5'
 
5' TAGATGCACCTGCCTGCCGC 3'
   ||||||||||||||||||||
3' ATCTACGTGGACGGACGGCG 5'
    
Genomic Alignments Position Identity Strand Genomic Target
laevis 9.2 chr3L:108458434..108458453 20/20 Antisense target smad6.L
chr3S:35721497..35721516 20/20 Sense target smad6.S
chr8L:113265171..113265190 18/20 Antisense off-target pea15.L
chr1L:75637238..75637257 17/20 Antisense off-target slc4a4.L
chr4L:128690069..128690088 16/20 Antisense off-target plxna1.L
chr4S:47240760..47240779 18/20 Antisense unknown
chr2S:49492619..49492638 16/20 Antisense unknown
chr7S:45567541..45567560 16/20 Antisense unknown
chr9_10S:43297972..43297991 16/20 Sense unknown
tropicalis 10.0 Chr3:117294662..117294681 20/20 Antisense smad6
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020                        
???morpholino.displaySummary.papersRecent??? Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022          
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