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XB-GRNA-23659145
gRNA Name:
smad6 gRNA1
Synonyms: smad6_g1
Genomic Target: smad6
PAM Sequence: AGG
Sequence: 5' GAATCTGGAAAACCGGAGTG 3'
Synonyms: smad6_g1
Genomic Target: smad6
PAM Sequence: AGG
Sequence: 5' GAATCTGGAAAACCGGAGTG 3'
![Run BLAST on sequence](img/icons/rocket.gif)
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 20/20 | 18/20 | 19/20 | ||||
genome
gRNA
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5' CACTCCGGTTTTCCAGATTC 3' |||||||||||||||||||| 3' GTGAGGCCAAAAGGTCTAAG 5' |
5' CGCTCCGGTTTTCCAGATTG 3' | ||||||||||||||||| 3' GTGAGGCCAAAAGGTCTAAG 5' |
5' CACTCCGGTTTTCCAGATTG 3'
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3' GTGAGGCCAAAAGGTCTAAG 5' |
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Genomic Alignments ![]() |
Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr3S:35721634..35721653 | 19/20 | Antisense | target | smad6.S | ||
chr3L:108458297..108458316 | 18/20 | Sense | target | smad6.L | |||
chr5L:35503969..35503988 | 17/20 | Sense | off-target | LOC108716608 | |||
chr1S:148037591..148037610 | 17/20 | Antisense | unknown | ||||
chr3L:111262381..111262400 | 17/20 | Antisense | unknown | ||||
chr3S:114863444..114863463 | 17/20 | Sense | unknown | ||||
chr6L:121937257..121937276 | 16/20 | Antisense | unknown | tropicalis 10.0 | Chr3:117294525..117294544 | 20/20 | Sense | target | smad6 |
Chr3:140104489..140104508 | 17/20 | Antisense | off-target | plppr2 | |||
Chr3:140104489..140104508 | 17/20 | Antisense | off-target | tmem205 | |||
Chr10:42719203..42719222 | 17/20 | Sense | off-target | LOC101734097 | |||
Chr2:168018964..168018983 | 16/20 | Sense | unknown | ||||
Chr3:55616503..55616522 | 16/20 | Antisense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 ![]() |
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???morpholino.displaySummary.papersRecent??? |
Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022 ![]() |
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???morpholino.displaySummary.papersAll??? |