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Summary Attributions Wiki
XB-GRNA-23659137
gRNA Name: dnm2 gRNA1
Synonyms: dnm2_g1, Xl.dnm2 (L+S), Xt.dnm2
Genomic Target: dnm2

PAM Sequence: GGG
Sequence: 5' CGGAGACCGAGTGCAGCCAT 3'



Genomic Target tropicalis laevis.L
Identities 20/20 20/20
genome
gRNA
 
5' ATGGCTGCACTCGGTCTCCG 3'
   ||||||||||||||||||||
3' TACCGACGTGAGCCAGAGGC 5'
 
5' ATGGCTGCACTCGGTCTCCG 3'
   ||||||||||||||||||||
3' TACCGACGTGAGCCAGAGGC 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr3L:148294281..148294300 20/20 Sense target dnm2.L
Chr3S:123851923..123851942 20/20 Sense off-target LOC108703474
tropicalis 10.0 Chr3:138759408..138759427 20/20 Sense target dnm2
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
???morpholino.displaySummary.papersRecent??? Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis., Dev Cell 2025     
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