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XB-GRNA-23659134
gRNA Name:
amer3 gRNA1
Synonyms:
Genomic Target: amer3
PAM Sequence: GGG
Sequence: 5' GTCAGGGACATTTCAAGGTG 3'
Synonyms:
Genomic Target: amer3
PAM Sequence: GGG
Sequence: 5' GTCAGGGACATTTCAAGGTG 3'
Genomic Target | tropicalis | laevis.L | |||||
Identities | 20/20 | 19/20 | |||||
genome
gRNA
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5' CACCTTGAAATGTCCCTGAC 3' |||||||||||||||||||| 3' GTGGAACTTTACAGGGACTG 5' |
5' CACCTTGAAATGTCCCAGAC 3'
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3' GTGGAACTTTACAGGGACTG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 10.1 | Chr5L:136522920..136522939 | 19/20 | Sense | target | amer3.L | ||
Chr3L:14860359..14860378 | 17/20 | Sense | off-target | iqsec3.L | |||
Chr6S:100394960..100394979 | 17/20 | Sense | off-target | atp6v1h.S | |||
Chr1L:91113415..91113434 | 16/20 | Antisense | off-target | ptpn13.L | |||
Chr9_10S:112315926..112315945 | 16/20 | Sense | off-target | LOC121399004 | |||
Chr5S:114497675..114497694 | 19/20 | Sense | unknown | ||||
Chr8S:21857679..21857698 | 18/20 | Sense | unknown | ||||
Chr9_10L:8132093..8132112 | 18/20 | Antisense | unknown | ||||
Chr6S:30956553..30956572 | 18/20 | Antisense | unknown | ||||
Chr6S:69355518..69355537 | 17/20 | Antisense | unknown | ||||
Chr9_10S:112593418..112593437 | 16/20 | Sense | unknown | ||||
Chr1S:78231139..78231158 | 16/20 | Sense | unknown | ||||
Chr5L:104525365..104525384 | 16/20 | Antisense | unknown | tropicalis 10.0 | Chr5:126986653..126986672 | 20/20 | Sense | target | amer3 |
Chr2:154076248..154076267 | 18/20 | Antisense | off-target | medag | |||
Chr2:154076248..154076267 | 18/20 | Antisense | off-target | LOC105946588 | |||
Chr9:52586818..52586837 | 16/20 | Antisense | off-target | nbeal1 | |||
Chr6:26223466..26223485 | 18/20 | Antisense | unknown | ||||
Chr6:93718957..93718976 | 17/20 | Sense | unknown | ||||
Chr2:99879091..99879110 | 16/20 | Antisense | unknown | ||||
Chr7:41878363..41878382 | 16/20 | Antisense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 |
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???morpholino.displaySummary.papersRecent??? |
Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022 |
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???morpholino.displaySummary.papersAll??? |