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XB-GRNA-23659128
gRNA Name:
pten gRNA1
Synonyms:
Genomic Target: pten
PAM Sequence: AGG
Sequence: 5' GAGTTACAATTCCCAGCCAA 3'
Synonyms:
Genomic Target: pten
PAM Sequence: AGG
Sequence: 5' GAGTTACAATTCCCAGCCAA 3'
Genomic Target | tropicalis | laevis.S | |||||
Identities | 20/20 | 19/20 | |||||
genome
gRNA
|
5' TTGGCTGGGAATTGTAACTC 3' |||||||||||||||||||| 3' AACCGACCCTTAACATTGAG 5' |
5' TTGGCTGGGAATTGTGACTC 3'
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3' AACCGACCCTTAACATTGAG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr7S:5633980..5633999 | 19/20 | Antisense | target | pten.S | ||
chr1L:162032233..162032252 | 16/20 | Antisense | off-target | gin1.L | |||
chr2S:83887492..83887511 | 16/20 | Antisense | off-target | atp2a3.S | |||
chr9_10S:36918761..36918780 | 16/20 | Sense | off-target | LOC108702845 [provisional:tbcd] | |||
chr8S:2437933..2437952 | 19/20 | Sense | unknown | ||||
chr7S:1046607..1046626 | 16/20 | Sense | unknown | ||||
chr8L:52619959..52619978 | 16/20 | Sense | unknown | tropicalis 10.0 | Chr7:12950899..12950918 | 20/20 | Antisense | target | pten |
Chr2:12078692..12078711 | 18/20 | Antisense | off-target | erg | |||
Chr8:24951066..24951085 | 18/20 | Sense | off-target | LOC108645248 | |||
Chr8:31383378..31383397 | 18/20 | Sense | off-target | LOC108645184 | |||
Chr5:17228233..17228252 | 17/20 | Antisense | off-target | haao | |||
Chr3:12974974..12974993 | 17/20 | Sense | off-target | nmur2 | |||
Chr9:83535720..83535739 | 17/20 | Sense | off-target | racgap1.2 | |||
Chr10:30763645..30763664 | 17/20 | Sense | off-target | LOC108644523 | |||
Chr9:73410006..73410025 | 16/20 | Sense | off-target | tanc1 | |||
Chr1:77867669..77867688 | 16/20 | Sense | off-target | ccser1 | |||
Chr8:71665344..71665363 | 16/20 | Antisense | off-target | gabbr1 | |||
Chr10:14569389..14569408 | 20/20 | Antisense | unknown | ||||
Chr8:17392675..17392694 | 18/20 | Sense | unknown | ||||
Chr3:43204382..43204401 | 17/20 | Antisense | unknown | ||||
Chr8:135702276..135702295 | 16/20 | Sense | unknown | ||||
Publications | |||||||
???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020 |
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???morpholino.displaySummary.papersRecent??? | |||||||
???morpholino.displaySummary.papersAll??? |