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Summary Attributions Wiki
XB-GRNA-23659122

gRNA Name: abca4 gRNA1
Synonyms: abca4_g1
Genomic Target: abca4

Sequence: 5' GAGGAAACTCTTGGGGACTT 3'



Genomic Target tropicalis laevis.L
Identities 20/20 19/20
genome
gRNA
5' AAGTCCCCAAGAGTTTCCTC 3'
   ||||||||||||||||||||
3' TTCAGGGGTTCTCAAAGGAG 5'
5' AGGTCCCCAAGAGTTTCCTC 3'
   | ||||||||||||||||||
3' TTCAGGGGTTCTCAAAGGAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4L:99731981..99732000 19/20 Sense target abca4.L
Chr8L:87275226..87275245 18/20 Sense off-target nin.L
Chr3L:17310425..17310444 18/20 Antisense off-target fkbp4.L
Chr5L:59975736..59975755 18/20 Sense off-target tarbp1.L
Chr8S:82311513..82311532 18/20 Sense off-target ppp1r10.S
Chr5S:143114481..143114500 17/20 Sense off-target LOC108717526
Chr1S:189564678..189564697 16/20 Sense off-target malt1.S
Chr2L:108106582..108106601 18/20 Sense unknown
Chr5L:106505687..106505706 18/20 Antisense unknown
Chr3S:44307711..44307730 17/20 Antisense unknown
tropicalis 10.0 Chr4:103734204..103734223 20/20 Sense target abca4
Chr9:5839383..5839402 18/20 Antisense off-target hsd3b7
Chr6:37248587..37248606 18/20 Sense off-target sostdc1
Chr9:33553997..33554016 16/20 Antisense unknown
Chr9:33555083..33555102 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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