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XB-GRNA-23659116
gRNA Name:
ezh2 gRNA2
Synonyms: ezh2_g2
Genomic Target: ezh2
PAM Sequence: TGG
Sequence: 5' AGCAGTTTGGATTCACAGAA 3'
Synonyms: ezh2_g2
Genomic Target: ezh2
PAM Sequence: TGG
Sequence: 5' AGCAGTTTGGATTCACAGAA 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 20/20 | 19/20 | ||||
genome
gRNA
|
5' TTCTGTGAATCCAAACTGCT 3' |||||||||||||||||||| 3' AAGACACTTAGGTTTGACGA 5' |
5' TTCTGTGAATCCAAACTGCT 3' |||||||||||||||||||| 3' AAGACACTTAGGTTTGACGA 5' |
5' TTCTCTGAATCCAAACTGCT 3'
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3' AAGACACTTAGGTTTGACGA 5' |
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Genomic Alignments  |
Position | Identity | Strand | Genomic Target | |||
| laevis 10.1 | Chr6L:58354345..58354364 | 20/20 | Antisense | target | ezh2.L | ||
| Chr6S:48286572..48286591 | 19/20 | Antisense | target | ezh2.S | |||
| Chr1S:195409355..195409374 | 18/20 | Sense | off-target | setbp1.S | |||
| Chr5S:119179802..119179821 | 18/20 | Antisense | off-target | dzip1l.S | |||
| Chr4S:67925198..67925217 | 17/20 | Antisense | off-target | dab1.S | |||
| Chr3L:71503778..71503797 | 17/20 | Sense | off-target | zcrb1.L | |||
| Chr9_10L:33333692..33333711 | 17/20 | Antisense | off-target | LOC108701017 | |||
| Chr5S:41282811..41282830 | 16/20 | Antisense | off-target | strn.S | |||
| Chr1L:18891804..18891823 | 16/20 | Antisense | off-target | reep1.L | |||
| Chr1L:14776662..14776681 | 16/20 | Antisense | off-target | adra1d.L | |||
| Chr3L:102327389..102327408 | 16/20 | Sense | off-target | LOC108710294 | |||
| Chr7S:89442893..89442912 | 19/20 | unknown | |||||
| Chr7L:119394130..119394149 | 18/20 | unknown | |||||
| Chr2S:83810391..83810410 | 17/20 | unknown | |||||
| Chr2S:134142998..134143017 | 16/20 | unknown | tropicalis 10.0 | Chr6:55170690..55170709 | 20/20 | Antisense | ezh2 |
| Chr3:107953805..107953824 | 18/20 | Sense | fes | ||||
| Chr3:80054768..80054787 | 18/20 | Sense | sema3e | ||||
| Chr2:20352280..20352299 | 18/20 | Antisense | tmprss15 | ||||
| Chr9:19979482..19979501 | 18/20 | Antisense | arhgap17 | ||||
| Chr3:80054768..80054787 | 18/20 | Antisense | LOC108646168 | ||||
| Chr5:105731625..105731644 | 16/20 | Sense | mcf2l.2 | ||||
| Chr4:61559410..61559429 | 18/20 | ||||||
| Publications | |||||||
| ???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
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| ???morpholino.displaySummary.papersRecent??? | |||||||
| ???morpholino.displaySummary.papersAll??? | |||||||
