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Summary Attributions Wiki
XB-GRNA-23659101
gRNA Name: mtnr1a gRNA3
Synonyms: Xtmtnr1a T3, T3
Genomic Target: mtnr1a

Sequence: 5' GGGTTATTGGGAGGATAAAG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 18/20
genome
gRNA
 5' CTTTATCCTCCCAATAACCA 3' ||||||||||||||||||| 3' GAAATAGGAGGGTTATTGGG 5' 5' CTTTATTCTCCCAATAACCG 3' |||||| |||||||||||| 3' GAAATAGGAGGGTTATTGGG 5'
Genomic Alignments Position Identity Strand Genomic Target
laevis 9.2 chr1S:32911857..32911876 18/20 Antisense target mtnr1a.S
chr9_10L:16478701..16478720 18/20 Antisense off-target gnas.L
chr1L:185320577..185320596 17/20 Sense off-target ak6.L
chr8L:49006546..49006565 16/20 Antisense off-target arhgap35.L
chr3L:77291976..77291995 18/20 unknown
chr2S:127612913..127612932 16/20 unknown
tropicalis 10.0 Chr1:43801824..43801843 19/20 Antisense mtnr1a
Chr10:2999670..2999689 18/20 Antisense gnas
Chr5:4786992..4787011 18/20 Antisense lrfn2
Chr8:11847073..11847092 17/20 Sense ntng2
Chr1:14028325..14028344 19/20
Chr3:140186880..140186899 18/20
Publications
First: CRISPR/Cas9 mediated mutation of the mtnr1a melatonin receptor gene causes rod photoreceptor degeneration in developing Xenopus tropicalis., Sci Rep 2020                                    
Most recent:
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