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Summary Images Attributions Wiki Source

Antibody Name: Akt1 Ab10

Common Name: Phospho-Akt

Synonyms: 4060, Phospho-Akt (Ser473), Phospho-Akt (Ser473) (D9E)

Gene: akt2 , akt1

Clone Type: Monoclonal
Source: Cell Signaling Technology

Clone Number D9E
Purification Affinity Purification
Isotype IgG
Host Organism rabbit
Xenopus Reactivity X. laevis
Non-Xenopus Reactivity human, monkey, mouse, rat, zebrafish
Description: Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. Cite: Cell Signaling Technology, #4060
Type polypeptide
Post Translational Modifications None
Source Organism Other
Reported Usage
western blot 1:2000
immunoprecipitation 1:50
immunohistochemistry 1:50 - 1:200
immunofluorescence 1:400 - 1:800
flow cytometry 1:100 - 1:400
First: Metalloprotease ADAM9 cleaves ephrin-B ligands and differentially regulates Wnt and mTOR signaling downstream of Akt kinase in colorectal cancer cells., J Biol Chem 2022                              
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