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Summary Images Attributions Wiki Source
XB-ANTIBODY-23659594

Antibody Name: Col1a1 Ab20

Common Name: SP1.D8

RRID: AB_528438
Synonyms: Collagen, Type I pro-peptide, collagen type I alpha 1 chain

Antigens
Gene: col1a1

Clone Type: Monoclonal
Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number
Purification Affinity Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity chicken, cow, dog, human, mouse, rabbit, rat, zebrafish
Description: This MAB recognizes pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM, and does not cross react with Type III collagen propeptide. SP1.D8 was deposited to the DSHB by H. Furthmayr, Stanford University. Citation:(DSHB Cat# SP1.D8, RRID:AB_528438)
Immunogen
Name collagen
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description CNBr cleavage of pro-collagen type I from dermatosparactice sheep. The N-terminal propeptide extends from a.a 23-161. Epitope Location or Sequence: N-terminal residues in Type I collagen. Immunogen Sequence: Amino propeptide amino acids 1-9
Sequence
Reported Usage
electrophoretic mobility shift assay
immunofluorescence
immunohistochemistry
western blot
Used For Epitope Tagging Yes
Publications
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