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Antibody Name: Rho Ab10 Common Name: Rhodopsin RRID: AB_477464 Synonyms: 1D4, R5403, Rhodopsin 1D4 Antigens XAO: retina, photoreceptor layer, retinal cone cell Clone Type: Monoclonal Source: Sigma-Aldrich

Antibody
Clone Number	1D4
Purification	Affinity Purification
Isotype	IgG1
Host Organism	mouse
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity	human, rat
Description:	Sigma-Aldrich Cat# R5403. May only bind artificial constructs of mammalian rhodopsin, pay particular attention to details of experiments using this antibody
Immunogen
Name	rho
Type	polypeptide
Post Translational Modifications	None
Source Organism	Other
Description	Fimmuogen is a bovine rhodopsin. By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
Sequence
Reported Usage
immunohistochemistry
Publications
???displayAntibody.papersFirst???	Noggin 1 overexpression in retinal progenitors affects bipolar cell generation., Int J Dev Biol 2016
???displayAntibody.papersRecent???	Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa., J Neurosci 2017
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