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Antibody Name: BrdU Ab9

Common Name: Bromodeoxyuridine

RRID: AB_2536432
Synonyms: B35128, MoBu-1, MoBu1

Other: BrdU

Clone Type: Monoclonal
Source: Thermo Fisher

Clone Number MoBu-1
Isotype IgG
Host Organism mouse
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity human, monkey
Description: Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. The MoBU-1 clone has a high specificity for BrdU, in addition to no cross-reactivity with EdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Citation:(Thermo Fisher Scientific Cat# B35128, RRID:AB_2536432).
Name BrdU
Type nucleic acid
Post Translational Modifications None
Source Organism mouse
Description Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication.
Reported Usage
First: aPKC phosphorylates p27Xic1, providing a mechanistic link between apicobasal polarity and cell-cycle control., Dev Cell 2014                            
Most recent: Foxm1 regulates neural progenitor fate during spinal cord regeneration., EMBO Rep 2021                          
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