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Summary Images Attributions Wiki Source
XB-ANTIBODY-17251818

Antibody Name: Cdc45 Ab2

Common Name:

RRID:
Synonyms:
Antigens
Clone Type: unknown
Source:

Antibody
Clone Number
Purification Affinity Purification
Isotype isotype unknown
Host Organism sheep
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity
Description: Generated in Blow's lab (2011). Cdc45 antibodies were raised in sheep against full-length His6-tagged X. laevis recombinant proteins expressed and purified from Escherichia coli (RosettaTM(DE3)pLysS, Novagen) using Ni2+-NTA affinity chromatography. Antibodies were used for immunoblotting at 1:1000 dilutions.
Immunogen
Name cdc45
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description Cdc45 antibodies were raised in sheep against full-length His6-tagged X. laevis recombinant proteins
Sequence
Reported Usage
western blot 1:1000
Publications
???displayAntibody.papersFirst??? MCM2-7 form double hexamers at licensed origins in Xenopus egg extract., J Biol Chem 2011                  
???displayAntibody.papersRecent??? The structural mechanism of dimeric DONSON in replicative helicase activation., Mol Cell 2023                                
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