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Antibody Name: Hnf1a Ab1 Common Name: XAD5 RRID: Synonyms: XAD5, XLFB1, XLFB1a, XLFB1b Antigens Gene: hnf1a XAO: alimentary system, liver, gall bladder, epithelium Clone Type: Monoclonal Source: Ryffel Lab

Antibody
Clone Number	XAD5
Purification
Isotype	isotype unknown
Host Organism	mouse
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity
Description:	Generated by Ryffel lab in 1993
Immunogen
Name	Hnf1a
Type	polypeptide
Post Translational Modifications	None
Source Organism	frog
Description	The 3'-terminal EcoRI fragment of the al cDNA clone, encoding the activation domain of XLFBla spanning from the EcoRI site at position 1058 (see Fig. 1) to the EcoRI site in the cloning adaptor, was ligated into the EcoRI site of prokaryotic expression vector pGEX-3X (Pharmacia). The fusion protein was purified from isopropyl-o-D-thiogalactopyranoside (IPTG)-induced bacteria on a glutathione-Sepharose 4B column (Pharmacia) as described by the manufacturer. AA 1-605
Sequence
Reported Usage
western blot
immunohistochemistry
Publications
First:	Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis., Mol Cell Biol 1993
Most recent:	Mesoderm and endoderm differentiation in animal cap explants: identification of the HNF4-binding site as an activin A responsive element in the Xenopus HNF1alpha promoter., Development 1996
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