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Antibody Name: Smad1 Ab7

Common Name: phospho-smad1/5/9

RRID: AB_331671
Synonyms: #9511, 9511, 9511L, phospho-smad1/5/8

Gene: smad9 , smad1

Clone Type: Polyclonal
Source: Cell Signaling Technology

Clone Number
Isotype IgG
Host Organism rabbit
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity human
Description: Supplied by Cell Signaling Technology, Cat #9511. Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467)
Name phospho-smad1/5/9
Type synthetic sequence
Post Translational Modifications Phosphorylated
Source Organism human
Description Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Antibody detects endogenous levels of Smad1 only when dually phosphorylated at serine 463 and serine 465, as well as Smad5 and Smad8 only when phosphorylated at the equivalent sites. The antibody does not cross-react with other Smad-related proteins.
Reported Usage
western blot use at 1:1000-1:5000
immunofluorescence MEMFA 1-2 hrs @ RT, post-fix Dents, use at 1:500
First: Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike)., Dev Dyn 2005                
Most recent: BMP signaling is enhanced intracellularly by FHL3 controlling WNT-dependent spatiotemporal emergence of the neural crest., Cell Rep 2021                          
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