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XB-ANTIBODY-14579139
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Antibody Name:
Smad1 Ab7
Common Name: phospho-smad1/5/9 RRID: AB_331671 Synonyms: #9511, 9511, 9511L, phospho-smad1/5/8
Source: Cell Signaling Technology |
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| Antibody | |
| Clone Number | |
| Purification | |
| Isotype | IgG |
| Host Organism | rabbit |
| Xenopus Reactivity | X. tropicalis, X. laevis |
| Non-Xenopus Reactivity | human |
| Description: | Supplied by Cell Signaling Technology, Cat #9511. Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) |
| Immunogen | |
| Name | phospho-smad1/5/9 |
| Type | synthetic sequence |
| Post Translational Modifications | Phosphorylated |
| Source Organism | human |
| Description | Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Antibody detects endogenous levels of Smad1 only when dually phosphorylated at serine 463 and serine 465, as well as Smad5 and Smad8 only when phosphorylated at the equivalent sites. The antibody does not cross-react with other Smad-related proteins. |
| Sequence | |
| Reported Usage | |
| immunohistochemistry | |
| western blot | use at 1:1000-1:5000 |
| immunofluorescence | MEMFA 1-2 hrs @ RT, post-fix Dents, use at 1:500 |
| Publications | |
| First: |
Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike)., Dev Dyn 2005  |
| Most recent: |
BMP signaling is enhanced intracellularly by FHL3 controlling WNT-dependent spatiotemporal emergence of the neural crest., Cell Rep 2021  |
| View All Papers | |
