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XB-ANTIBODY-18258718
Antibody Name:
Tcof1 Ab1
Common Name: treacle RRID: Synonyms: xtreacle
Source: |
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Antibody | |
Clone Number | |
Purification | |
Isotype | IgG |
Host Organism | chicken |
Xenopus Reactivity | X. tropicalis, X. laevis |
Non-Xenopus Reactivity | |
Description: | The 5â² end of xTcof1 cDNA was amplified by PCR using BV1180 (5â²-ACTCAGATCCCATGGAGGAAGTGGAGGGATCC-3â²) and BV1181 (5â²-TCCAGAGCTCTCGAGTCACTGTGCAATCACAGGTGT-3â²) and subcloned into the NcoI and XhoI sites of pPROEX HTa vector. The E. coli-expressed poly-histidine-tagged protein, which included amino acids 1â307 of Xenopus treacle (xtreacle), was purified using Ni-NTA agarose (Qiagen, Inc.) and sent to Sigma for antibody production in chicken. |
Immunogen | |
Name | tcof1 |
Type | polypeptide |
Post Translational Modifications | None |
Source Organism | chicken |
Description | |
Sequence | |
Reported Usage | |
immunofluorescence | |
western blot | |
Publications | |
???displayAntibody.papersFirst??? |
Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene product., Gene 2005 |
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