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Antibody Name: Neuronal Ab1 Common Name: zn-12 RRID: AB_531908 Synonyms: Antigens XAO: muscle, neuron Clone Type: Monoclonal Source: Developmental Studies Hybridoma Bank

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Antibody
Clone Number	zn-12
Purification
Isotype	IgG1
Host Organism	mouse
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity	chicken, zebrafish
Description:	Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface of many but not all neurons, fibers and neuropil, muscle cells and receptor cells. zn-12 was deposited to the DSHB by B. Trevarrow, Institute of Neuroscience, University of Oregon. Citation: (DSHB Cat# zn-12, RRID:AB_531908). Please send all citations to the DSHB.
Immunogen
Name	brain membranes
Type	homogenized tissue
Post Translational Modifications	None
Source Organism	Other
Description	source organism: zebrafish. Zn-12 recognizes both sulfated and non-sulfated HNK-1 epitopes.
Sequence
Reported Usage
immunohistochemistry	1:40, Dent's Fixative
western blot
immunofluorescence	1:40, Dent's Fixative
immunoprecipitation
Publications
???displayAntibody.papersFirst???	Organization of hindbrain segments in the zebrafish embryo., Neuron 1990
???displayAntibody.papersRecent???	Regulation of neurogenesis by Fgf8a requires Cdc42 signaling and a novel Cdc42 effector protein., Dev Biol 2013
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