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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus., Blitz IL., J Vis Exp. February 1, 2018; (132):


Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function., Tu F, Sedzinski J, Ma Y, Marcotte EM, Wallingford JB., J Cell Sci. January 29, 2018; 131 (3):             


RAPGEF5 Regulates Nuclear Translocation of β-Catenin., Griffin JN, Del Viso F, Duncan AR, Robson A, Hwang W, Kulkarni S, Liu KJ, Khokha MK., Dev Cell. January 22, 2018; 44 (2): 248-260.e4.                                                


Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas., Sakane Y, Iida M, Hasebe T, Fujii S, Buchholz DR, Ishizuya-Oka A, Yamamoto T, Suzuki KT., Biol Open. January 22, 2018; 7 (1):             


An Intracellular Ammonium Transporter Is Necessary for Replication, Differentiation, and Resistance to Starvation and Osmotic Stress in Trypanosoma cruzi., Cruz-Bustos T, Potapenko E, Storey M, Docampo R., mSphere. January 17, 2018; 3 (1):                   


CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis., Naert T, Vleminckx K., Methods Mol Biol. January 1, 2018; 1726 177-193.


CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis., Deniz E, Mis EK, Lane M, Khokha MK., Methods Mol Biol. January 1, 2018; 1865 163-174.


Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors., Naert T, Vleminckx K., Methods Mol Biol. January 1, 2018; 1865 147-161.


How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique., Tadjuidje E, Cha SW., Methods Mol Biol. January 1, 2018; 1865 105-117.


A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair., Suzuki KT, Sakane Y, Suzuki M, Yamamoto T., Methods Mol Biol. January 1, 2018; 1865 91-103.


Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis., Naert T, Vleminckx K., Methods Mol Biol. January 1, 2018; 1865 67-82.


Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology., Van Nieuwenhuysen T, Vleminckx K., Methods Mol Biol. January 1, 2018; 1865 55-65.


Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering., Naert T, Vleminckx K., Methods Mol Biol. January 1, 2018; 1865 33-54.


Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus., Sempou E, Lakhani OA, Amalraj S, Khokha MK., Front Physiol. January 1, 2018; 9 1705.              


BATCH-GE: Analysis of NGS Data for Genome Editing Assessment., Steyaert W, Boel A, Coucke P, Willaert A., Methods Mol Biol. January 1, 2018; 1865 83-90.


CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing., Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ., Nat Commun. December 8, 2017; 8 (1): 2024.        


Targeted Base Editing via RNA-Guided Cytidine Deaminases in Xenopus laevis Embryos., Park DS, Yoon M, Kweon J, Jang AH, Kim Y, Choi SC., Mol Cells. November 30, 2017; 40 (11): 823-827.    


Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature., Daste F, Walrant A, Holst MR, Gadsby JR, Mason J, Lee JE, Brook D, Mettlen M, Larsson E, Lee SF, Lundmark R, Gallop JL., J Cell Biol. November 6, 2017; 216 (11): 3745-3765.                


Angiopoietin-like 4 Is a Wnt Signaling Antagonist that Promotes LRP6 Turnover., Kirsch N, Chang LS, Koch S, Glinka A, Dolde C, Colozza G, Benitez MDJ, De Robertis EM, Niehrs C., Dev Cell. October 9, 2017; 43 (1): 71-82.e6.                                


Mutation of the inhibitory ethanol site in GABAA ρ1 receptors promotes tolerance to ethanol-induced motor incoordination., Blednov YA, Borghese CM, Ruiz CI, Cullins MA, Da Costa A, Osterndorff-Kahanek EA, Homanics GE, Harris RA., Neuropharmacology. September 1, 2017; 123 201-209.


Two-Element Transcriptional Regulation in the Canonical Wnt Pathway., Kim K, Cho J, Hilzinger TS, Nunns H, Liu A, Ryba BE, Goentoro L., Curr Biol. August 7, 2017; 27 (15): 2357-2364.e5.                


An analysis of MyoD-dependent transcription using CRISPR/Cas9 gene targeting in Xenopus tropicalis embryos., McQueen C, Pownall ME., Mech Dev. August 1, 2017; 146 1-9.          


Acetylcholinesterase plays a non-neuronal, non-esterase role in organogenesis., Pickett MA, Dush MK, Nascone-Yoder NM., Development. August 1, 2017; 144 (15): 2764-2770.                    


High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0 Xenopus., Aslan Y, Tadjuidje E, Zorn AM, Cha SW., Development. August 1, 2017; 144 (15): 2852-2858.      


Modeling Dominant and Recessive Forms of Retinitis Pigmentosa by Editing Three Rhodopsin-Encoding Genes in Xenopus Laevis Using Crispr/Cas9., Feehan JM, Chiu CN, Stanar P, Tam BM, Ahmed SN, Moritz OL., Sci Rep. July 31, 2017; 7 (1): 6920.              


The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility., Fin L, Bergamin G, Steiner RA, Hughes SM., Sci Rep. July 7, 2017; 7 (1): 4858.        


Id genes are essential for early heart formation., Cunningham TJ, Yu MS, McKeithan WL, Spiering S, Carrette F, Huang CT, Bushway PJ, Tierney M, Albini S, Giacca M, Mano M, Puri PL, Sacco A, Ruiz-Lozano P, Riou JF, Umbhauer M, Duester G, Mercola M, Colas AR., Genes Dev. July 1, 2017; 31 (13): 1325-1338.                


Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling., Tandon P, Conlon F, Furlow JD, Horb ME., Dev Biol. June 15, 2017; 426 (2): 325-335.      


Exploring the functions of nonclassical MHC class Ib genes in Xenopus laevis by the CRISPR/Cas9 system., Banach M, Edholm ES, Robert J., Dev Biol. June 15, 2017; 426 (2): 261-269.          


Distinct cis-acting regions control six6 expression during eye field and optic cup stages of eye formation., Ledford KL, Martinez-De Luna RI, Theisen MA, Rawlins KD, Viczian AS, Zuber ME., Dev Biol. June 15, 2017; 426 (2): 418-428.                        


Stomach curvature is generated by left-right asymmetric gut morphogenesis., Davis A, Amin NM, Johnson C, Bagley K, Ghashghaei HT, Nascone-Yoder N., Development. April 15, 2017; 144 (8): 1477-1483.                      


A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors., Bryant DM, Johnson K, DiTommaso T, Tickle T, Couger MB, Payzin-Dogru D, Lee TJ, Leigh ND, Kuo TH, Davis FG, Bateman J, Bryant S, Guzikowski AR, Tsai SL, Coyne S, Ye WW, Freeman RM, Peshkin L, Tabin CJ, Regev A, Haas BJ, Whited JL., Cell Rep. January 17, 2017; 18 (3): 762-776.                          


TALENs and CRISPR/Cas9 fuel genetically engineered clinically relevant Xenopus tropicalis tumor models., Naert T, Van Nieuwenhuysen T, Vleminckx K., Genesis. January 1, 2017; 55 (1-2):         


A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System., Sakane Y, Suzuki KT, Yamamoto T., Methods Mol Biol. January 1, 2017; 1630 189-203.


Shaping Chromatin in the Nucleus: The Bricks and the Architects., Sitbon D, Podsypanina K, Yadav T, Almouzni G., Cold Spring Harb Symp Quant Biol. January 1, 2017; 82 1-14.            


Foxn4 promotes gene expression required for the formation of multiple motile cilia., Campbell EP, Quigley IK, Kintner C., Development. December 15, 2016; 143 (24): 4654-4664.                                  


CRISPR: MODIFYING THE LIFE SCIENCE LANDSCAPE., Blow N., Biotechniques. November 1, 2016; 61 (5): 225-231.


A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing., Fu L, Wen L, Luu N, Shi YB., Sci Rep. October 17, 2016; 6 35488.                                


CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis., Naert T, Colpaert R, Van Nieuwenhuysen T, Dimitrakopoulou D, Leoen J, Haustraete J, Boel A, Steyaert W, Lepez T, Deforce D, Willaert A, Creytens D, Vleminckx K., Sci Rep. October 14, 2016; 6 35264.                          


Cooperation of the ER-shaping proteins atlastin, lunapark, and reticulons to generate a tubular membrane network., Wang S, Tukachinsky H, Romano FB, Rapoport TA., Elife. September 13, 2016; 5                         


ge-CRISPR - An integrated pipeline for the prediction and analysis of sgRNAs genome editing efficiency for CRISPR/Cas system., Kaur K, Gupta AK, Rajput A, Kumar M., Sci Rep. September 1, 2016; 6 30870.        


TTC25 Deficiency Results in Defects of the Outer Dynein Arm Docking Machinery and Primary Ciliary Dyskinesia with Left-Right Body Asymmetry Randomization., Wallmeier J, Shiratori H, Dougherty GW, Edelbusch C, Hjeij R, Loges NT, Menchen T, Olbrich H, Pennekamp P, Raidt J, Werner C, Minegishi K, Shinohara K, Asai Y, Takaoka K, Lee C, Griese M, Memari Y, Durbin R, Kolb-Kokocinski A, Sauer S, Wallingford JB, Hamada H, Omran H., Am J Hum Genet. August 4, 2016; 99 (2): 460-9.


Nodal signalling in Xenopus: the role of Xnr5 in left/right asymmetry and heart development., Tadjuidje E, Kofron M, Mir A, Wylie C, Heasman J, Cha SW., Open Biol. August 1, 2016; 6 (8):             


Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes., Blitz IL, Fish MB, Cho KW., Development. August 1, 2016; 143 (15): 2868-75.        


Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders., Shigeta M, Sakane Y, Iida M, Suzuki M, Kashiwagi K, Kashiwagi A, Fujii S, Yamamoto T, Suzuki KT., Genes Cells. July 1, 2016; 21 (7): 755-71.                


Research advances on animal genetics in China in 2015., Zhang B, Chen XF, Huang X, Yang X., Yi Chuan. June 20, 2016; 38 (6): 467-507.


The ciliopathy-associated CPLANE proteins direct basal body recruitment of intraflagellar transport machinery., Toriyama M, Lee C, Taylor SP, Duran I, Cohn DH, Bruel AL, Tabler JM, Drew K, Kelly MR, Kim S, Park TJ, Braun DA, Pierquin G, Biver A, Wagner K, Malfroot A, Panigrahi I, Franco B, Al-Lami HA, Yeung Y, Choi YJ, University of Washington Center for Mendelian Genomics, Duffourd Y, Faivre L, Rivière JB, Chen J, Liu KJ, Marcotte EM, Hildebrandt F, Thauvin-Robinet C, Krakow D, Jackson PK, Wallingford JB., Nat Genet. June 1, 2016; 48 (6): 648-56.                              


Nucleosome breathing and remodeling constrain CRISPR-Cas9 function., Isaac RS, Jiang F, Doudna JA, Lim WA, Narlikar GJ, Almeida R., Elife. April 28, 2016; 5           


Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number., Spadafora D, Kozhukhar N, Alexeyev MF., PLoS One. March 31, 2016; 11 (3): e0152705.              


Using Xenopus to study genetic kidney diseases., Lienkamp SS., Semin Cell Dev Biol. March 1, 2016; 51 117-24.    

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