XB-ART-8812Differentiation 2001 Mar 01;673:80-3.
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Mitochondrial ribosomal RNA in the germinal granules in Xenopus embryos revisited.
Germ cells of various animals contain a determinant that is called the germ plasm. In amphibians such as Xenopus laevis, the germ plasm is composed of mitochondria and electron dense germinal granules that are embedded in a fibrillar matrix. Previous reports indicated that one of the components of germinal granules was mitochondrial large and small ribosomal RNA (mtlrRNA and mtsrRNA). Utilizing a modified procedure for electron microscopy in situ hybridization, we investigated the distribution of these RNAs along with other components of the germ plasm in Xenopus laevis embryos. We found, that contrary to previous reports, the mtlrRNA and mtsrRNA were located in close vicinity to the germinal granules but were not major constituents of granules. The majority of the mtlrRNA and mtlsrRNAs was present inside the mitochondria and in the germ plasm matrix.
PubMed ID: 11428130
Article link: Differentiation
Species referenced: Xenopus laevis
Genes referenced: cat2 nanos1
Article Images: [+] show captions
|Fig. 1 Electron microscopy analysis of in situ hybridization with Xcat2 mRNA (A), mtlrRNA (B), and mtsrRNA (C) in 8 cell stage Xenopus embryos. Arrows point to the germinal granules. Mitochondria (M). Bar is equal to 400 nm|
|Fig. 2 Student’s T test statistical analysis of the label in electron microscopy in situ hybridization samples. Graph depicts the distribution of the grains in three different cellular compartments (mitochondria, germinal granules, matrix) for three different RNA probes (mtlrRNA, mtsrRNA, Xcat2 mRNA) in an area of 42.5 mm2. Each bar represents the average (with the standard deviation) number of grain counted in 4–9 independent samples. Numbers below the graph represent the P values. All the P values 0.05 are statistically significant. NSωstatistically not significant|