Strutz-Seebohm N et al. (2006), Additive regulation of GluR1 by stargazin and s...

XB-ART-685

Pflugers Arch 2006 Jun 01;4523:276-82. doi: 10.1007/s00424-005-0032-7.

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Additive regulation of GluR1 by stargazin and serum- and glucocorticoid-inducible kinase isoform SGK3.

Strutz-Seebohm N , Seebohm G , Korniychuk G , Baltaev R , Ureche O , Striegel M , Lang F .

Abstract
The serum- and glucocorticoid-inducible kinase isoform 3 (SGK3) and stargazin have both been shown to enhance the synaptic expression level of GluR1. The present study was performed to elucidate whether SGK3 and stargazin interact or are effective through different pathways in the regulation of GluR1. Proteins were expressed in Xenopus oocytes by injection of complementary RNA (cRNA) encoding GluR1, SGK isoforms, and/or stargazin. In oocytes expressing GluR1 6 days after cRNA injection, glutamate induced an inward current (IGlu), which was increased approximately fourfold following coexpression of SGK3. Coexpression of stargazin similarly enhanced IGlu. Coexpression of both SGK3 and stargazin stimulated the current by a factor of 15.5. Replacement of the serine by alanine at the only SGK consensus sequence (RXRXXS/T) in stargazin enhanced the efficacy of stargazin but did not prevent further stimulation of IGlu by additional coexpression of SGK3. Western blotting showed that stargazin accelerated membrane insertion of GluR1 protein leading to enhanced GluR1 plasma membrane protein abundance 2 days, but not 6 days, after cRNA injection, while SGK3 increased plasma membrane protein abundance 6 days after cRNA injection. In conclusion, SGK3 and stargazin regulate GluR1 independently, and thus, their effects on glutamate-induced currents are additive.

PubMed ID: 16485113
Article link: Pflugers Arch

Species referenced: Xenopus
Genes referenced: cacng2 gria1 sgk1 sgk3

References [+] :

Antonova, Rapid increase in clusters of presynaptic proteins at onset of long-lasting potentiation. 2001, Pubmed

Antonova, Rapid increase in clusters of presynaptic proteins at onset of long-lasting potentiation. 2001, Pubmed
Beattie, Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD. 2000, Pubmed
Boehmer, Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B. 2003, Pubmed , Xenbase
Boehmer, Properties and regulation of glutamine transporter SN1 by protein kinases SGK and PKB. 2003, Pubmed , Xenbase
Boehmer, Serum and glucocorticoid inducible kinases in the regulation of the cardiac sodium channel SCN5A. 2003, Pubmed , Xenbase
Chen, Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms. 2001, Pubmed
Chen, Stargazin differentially controls the trafficking of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate and kainate receptors. 2003, Pubmed , Xenbase
Chen, Impaired cerebellar synapse maturation in waggler, a mutant mouse with a disrupted neuronal calcium channel gamma subunit. 1999, Pubmed
Chetkovich, Phosphorylation of the postsynaptic density-95 (PSD-95)/discs large/zona occludens-1 binding site of stargazin regulates binding to PSD-95 and synaptic targeting of AMPA receptors. 2002, Pubmed
Choi, Phosphorylation of stargazin by protein kinase A regulates its interaction with PSD-95. 2002, Pubmed
Daw, PDZ proteins interacting with C-terminal GluR2/3 are involved in a PKC-dependent regulation of AMPA receptors at hippocampal synapses. 2001, Pubmed
Ehlers, Reinsertion or degradation of AMPA receptors determined by activity-dependent endocytic sorting. 2000, Pubmed
Embark, Regulation of KCNE1-dependent K(+) current by the serum and glucocorticoid-inducible kinase (SGK) isoforms. 2003, Pubmed , Xenbase
Firestone, Stimulus-dependent regulation of serum and glucocorticoid inducible protein kinase (SGK) transcription, subcellular localization and enzymatic activity. 2003, Pubmed
Friedrich, The serine/threonine kinases SGK2 and SGK3 are potent stimulators of the epithelial Na+ channel alpha,beta,gamma-ENaC. 2003, Pubmed , Xenbase
Gamper, K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. 2002, Pubmed , Xenbase
Garner, PDZ domains in synapse assembly and signalling. 2000, Pubmed
Hashimoto, Impairment of AMPA receptor function in cerebellar granule cells of ataxic mutant mouse stargazer. 1999, Pubmed
Kennedy, Signal-processing machines at the postsynaptic density. 2000, Pubmed
Kobayashi, Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. 1999, Pubmed
Kobayashi, Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. 2000, Pubmed
Lang, Regulation and physiological roles of serum- and glucocorticoid-induced protein kinase isoforms. 2001, Pubmed
Lang, Regulation of channels by the serum and glucocorticoid-inducible kinase - implications for transport, excitability and cell proliferation. 2003, Pubmed
Letts, The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit. 1998, Pubmed
Lin, Distinct molecular mechanisms and divergent endocytotic pathways of AMPA receptor internalization. 2000, Pubmed
Lüscher, Role of AMPA receptor cycling in synaptic transmission and plasticity. 2000, Pubmed
Malinow, LTP mechanisms: from silence to four-lane traffic. 2000, Pubmed
Man, Regulation of AMPA receptor-mediated synaptic transmission by clathrin-dependent receptor internalization. 2000, Pubmed
McCormick, Targeted disruption of the protein kinase SGK3/CISK impairs postnatal hair follicle development. 2004, Pubmed
Pearce, SGK1 regulation of epithelial sodium transport. 2003, Pubmed
Priel, Stargazin reduces desensitization and slows deactivation of the AMPA-type glutamate receptors. 2005, Pubmed , Xenbase
Sarkar, The "megaprimer" method of site-directed mutagenesis. 1990, Pubmed
Schnell, Direct interactions between PSD-95 and stargazin control synaptic AMPA receptor number. 2002, Pubmed
Seebohm, Identification of specific pore residues mediating KCNQ1 inactivation. A novel mechanism for long QT syndrome. 2001, Pubmed , Xenbase
Strutz, Kainate-binding proteins are rendered functional ion channels upon transplantation of two short pore-flanking domains from a kainate receptor. 2002, Pubmed , Xenbase
Strutz-Seebohm, Glucocorticoid adrenal steroids and glucocorticoid-inducible kinase isoforms in the regulation of GluR6 expression. 2005, Pubmed , Xenbase
Strutz-Seebohm, Regulation of GluR1 abundance in murine hippocampal neurones by serum- and glucocorticoid-inducible kinase 3. 2005, Pubmed , Xenbase
Tomita, Dynamic interaction of stargazin-like TARPs with cycling AMPA receptors at synapses. 2004, Pubmed , Xenbase
Verrey, SGK1: aldosterone-induced relay of Na+ transport regulation in distal kidney nephron cells. 2003, Pubmed , Xenbase
Wulff, Impaired renal Na(+) retention in the sgk1-knockout mouse. 2002, Pubmed , Xenbase
Yun, Concerted roles of SGK1 and the Na+/H+ exchanger regulatory factor 2 (NHERF2) in regulation of NHE3. 2003, Pubmed
Ziff, Recent excitement in the ionotropic glutamate receptor field. 1999, Pubmed