XB-ART-6381Mech Dev 2002 Nov 01;1191:121-5. doi: 10.1016/s0925-4773(02)00308-8.
Show Gene links Show Anatomy links
Neural expression of mouse Noelin-1/2 and comparison with other vertebrates.
Noelins are secreted glycoproteins with important developmental functions in frogs and birds. Here, we present the expression pattern of the mouse homolog of Noelin-1/2 at E8-10 of development and compare this pattern to other vertebrates. Expression was observed in the neural plate and neural crest, as well as in the cranial ganglia. Later, expression is prominent in brain tissue and in the zone of polarizing activity in the limb.
PubMed ID: 12385760
Article link: Mech Dev
Species referenced: Xenopus
Genes referenced: olfm1 snai2 twist1
Article Images: [+] show captions
|Fig. 4. Species comparison of Noelin-1/2 expression. (A) Dorsal view of a stage 22 frog embryo (approximately 10-somite stage) stained with Noelin-1/2 reveals transcripts in the developing trigeminal ganglia and spinal cord, but not in the neural crest (anterior is to the right). (B) A transverse section through the rostral trunk reveals staining in developing neuron populations (arrowheads; section is counterstained with Light Green). (C) Dorso-lateral view of a stage 20 frog embryo stained with the neural crest marker XSlug reveals the location of neural crest cells at this stage (neural crest masses: m, mandibular; h, hyoid; b, branchial). (D) A stage 28 frog embryo stained with Noelin-1/2. Staining is found in cranial ganglia, the pineal gland and in the spinal cord. Cranial neural crest streams do not express Noelin-1/2 (locations of streams are noted in (D) and (E) m, mandibular; h, hyoid; ba, anterior branchial, bp, posterior branchial). (E) The neural crest marker XTwist reveals neural crest cells migrating into the branchial arches. (F) A 10-somite chick embryo with Noelin expression in the migrating neural crest in the midbrain region and in the neural tube from the hindbrain through the open neural plate. (G) A stage 18 chick embryo stained with Noelin. Note the Noelin expression in the anterior region of the chick limb bud, as opposed to the mouse in (H) which has Noelin staining in the posterior limb bud. Chick photos are courtesy of M. Barembaum. (H) A mouse E10 embryo stained with Noelin-1/2 for comparison to the frog and chick patterns. The tail is curved toward the viewer, causing the anterior/posterior axis of the limb to appear backwards. cg, cement gland; e, eye; fl, forelimb; hl, hindlimb; IN, interneurons; mb, midbrain; MN, motor neurons; np, neural plate; o, olfactory placode; V, trigeminal ganglion; VII, geniculate ganglion; sc, spinal cord; asterisks denote otic vesicles.|