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Development
2024 May 01;1519:. doi: 10.1242/dev.202529.
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RFX6 regulates human intestinal patterning and function upstream of PDX1.
Sanchez JG
,
Rankin S
,
Paul E
,
McCauley HA
,
Kechele DO
,
Enriquez JR
,
Jones NH
,
Greeley SAW
,
Letourneau-Friedberg L
,
Zorn AM
,
Krishnamurthy M
,
Wells JM
.
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The gastrointestinal tract is complex and consists of multiple organs with unique functions. Rare gene mutations can cause congenital malformations of the human GI tract, although the molecular basis of these has been poorly studied. We identified a patient with compound-heterozygous mutations in RFX6 presenting with duodenal mal-rotation and atresia, implicating RFX6 in development of the proximalintestine. To identify how mutations in RFX6 impact intestinal patterning and function, we derived iPSCs from this patient to generate human intestinal organoids (HIOs). We identified that the duodenal HIOs and patient tissues had mixed regional identity, with gastric and ileal features. CRISPR-mediated correction of RFX6 restored duodenal identity. We then used gain- and loss-of-function and transcriptomic approaches in HIOs and Xenopus embryos to identify that PDX1 is a downstream transcriptional target of RFX6 required for duodenal development. However, RFX6 had additional PDX1-independent transcriptional targets involving multiple components of signaling pathways that are critical for establishing early regional identity in the GI tract. In summary, we have identified RFX6 as a key regulator in intestinal patterning that acts by regulating transcriptional and signaling pathways.
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