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STAR Protoc 2024 Mar 15;51:102830. doi: 10.1016/j.xpro.2023.102830.
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A fractionation-based protocol to investigate RNA solubility phase transition during Xenopus oocyte maturation.

Hwang H , Ma M , Yang J .

While RNAs are soluble in vitro, their solubility may be altered when incorporated into some protein complexes inside the cell. The solubility phase transition of RNAs is thus indicative of changes in the function and activity of RNAs. Here, we present a protocol for the assessment of RNA solubility phase transition during Xenopus oocyte maturation. We describe steps for sample preparation, cell fractionation, RNA extraction, real-time PCR, and analysis of the obtained results. For complete details on the use and execution of this protocol, please refer to Hwang et al. (2023).1.

PubMed ID: 38198279
PMC ID: PMC10820796
Article link: STAR Protoc
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ccna1 cep152 ddx25 dnd1 sybu wee2
GO keywords: oocyte maturation

Article Images: [+] show captions
References [+] :
Heasman, Fertilization of cultured Xenopus oocytes and use in studies of maternally inherited molecules. 1991, Pubmed, Xenbase