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PLoS Biol 2023 Aug 01;218:e3002261. doi: 10.1371/journal.pbio.3002261.
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Paracrine regulation of neural crest EMT by placodal MMP28.

Gouignard N , Bibonne A , Mata JF , Bajanca F , Berki B , Barriga EH , Saint-Jeannet JP , Theveneau E .

Epithelial-mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for neural crest EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of neural crest cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.

PubMed ID: 37590318
PMC ID: PMC10479893
Article link: PLoS Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: eef1a1 eya1 fn1 foxd3 foxi4 foxi4.2 itk krt12.4 mmp14 mmp28 pax3 psmd6 sf3b4 six1 snai1 snai2 sox10 sox2 sox8 sox9 twist1
GO keywords: cell migration [+]
Antibodies: GFP Ab15 GFP Ab28
Morpholinos: mmp28 MO1 mmp28 MO2

Article Images: [+] show captions
References [+] :
Akkers, Chromatin immunoprecipitation analysis of Xenopus embryos. 2012, Pubmed, Xenbase