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BMC Vet Res
2023 Feb 11;191:47. doi: 10.1186/s12917-023-03606-3.
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Host defence peptide LEAP2 contributes to antimicrobial activity in a mustache toad (Leptobrachium liui).
Chen J
,
Zhang CY
,
Chen JY
,
Seah RWX
,
Zhang L
,
Ma L
,
Ding GH
.
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BACKGROUND: The liver-expressed antimicrobial peptide 2 (LEAP2) is essential in host immunity against harmful pathogens and is only known to act as an extracellular modulator to regulate embryonic development in amphibians. However, there is a dearth of information on the antimicrobial function of amphibian LEAP2. Hence, a LEAP2 homologue from Leptobrachium liui was identified, characterized, and chemically synthesized, and its antibacterial activities and mechanisms were determined.
RESULTS: In this study, LEAP2 gene (Ll-LEAP2) cDNA was cloned and sequenced from the Chong'an Moustache Toad (Leptobrachium liui). The predicted amino acid sequence of Ll-LEAP2 comprises a signal peptide, a mature peptide, and a prodomain. From sequence analysis, it was revealed that Ll-LEAP2 belongs to the cluster of amphibian LEAP2 and displays high similarity to the Tropical Clawed Frog (Xenopus tropicalis)'s LEAP2. Our study revealed that LEAP2 protein was found in different tissues, with the highest concentration in the kidney and liver of L. liui; and Ll-LEAP2 mRNA transcripts were expressed in various tissues with the kidney having the highest mRNA expression level. As a result of Aeromonas hydrophila infection, Ll-LEAP2 underwent a noticeable up-regulation in the skin while it was down-regulated in the intestines. The chemically synthesized Ll-LEAP2 mature peptide was selective in its antimicrobial activity against several in vitro bacteria including both gram-positive and negative bacteria. Additionally, Ll-LEAP2 can kill specific bacteria by disrupting bacterial membrane and hydrolyzing bacterial gDNA.
CONCLUSIONS: This study is the first report on the antibacterial activity and mechanism of amphibian LEAP2. With more to uncover, the immunomodulatory functions and wound-healing activities of Ll-LEAP2 holds great potential for future research.
Almagro Armenteros,
SignalP 5.0 improves signal peptide predictions using deep neural networks.
2019, Pubmed
Almagro Armenteros,
SignalP 5.0 improves signal peptide predictions using deep neural networks.
2019,
Pubmed
Bo,
Antimicrobial activity and mechanisms of multiple antimicrobial peptides isolated from rockfish Sebastiscus marmoratus.
2019,
Pubmed
Bosch,
Antimicrobial Peptides-or How Our Ancestors Learned to Control the Microbiome.
2021,
Pubmed
Brown,
Cationic host defense (antimicrobial) peptides.
2006,
Pubmed
Chen,
The protection effect of LEAP-2 on the mudskipper (Boleophthalmus pectinirostris) against Edwardsiella tarda infection is associated with its immunomodulatory activity on monocytes/macrophages.
2016,
Pubmed
Chen,
Molecular characterization of cathelicidin in tiger frog (Hoplobatrachus rugulosus): Antimicrobial activity and immunomodulatory activity.
2021,
Pubmed
Chen,
Mudskipper (Boleophthalmus pectinirostris) Hepcidin-1 and Hepcidin-2 Present Different Gene Expression Profile and Antibacterial Activity and Possess Distinct Protective Effect against Edwardsiella tarda Infection.
2018,
Pubmed
Demori,
Peptides for Skin Protection and Healing in Amphibians.
2019,
Pubmed
,
Xenbase
Di Grazia,
The Frog Skin-Derived Antimicrobial Peptide Esculentin-1a(1-21)NH2 Promotes the Migration of Human HaCaT Keratinocytes in an EGF Receptor-Dependent Manner: A Novel Promoter of Human Skin Wound Healing?
2015,
Pubmed
Fan,
Brevinin-2PN, an antimicrobial peptide identified from dark-spotted frog (Pelophylax nigromaculatus), exhibits wound-healing activity.
2022,
Pubmed
Field,
The White-Nose Syndrome Transcriptome: Activation of Anti-fungal Host Responses in Wing Tissue of Hibernating Little Brown Myotis.
2015,
Pubmed
Hancock,
Modulating immunity as a therapy for bacterial infections.
2012,
Pubmed
Hanson,
The Drosophila Baramicin polypeptide gene protects against fungal infection.
2021,
Pubmed
Henriques,
Structural and functional analysis of human liver-expressed antimicrobial peptide 2.
2010,
Pubmed
Hocquellet,
Structure-activity relationship of human liver-expressed antimicrobial peptide 2.
2010,
Pubmed
Ishige,
Characterization and expression of non-polymorphic liver expressed antimicrobial peptide 2: LEAP-2 in the Japanese quail, Coturnix japonica.
2016,
Pubmed
Jiang,
Molecular characterization of a MOSPD2 homolog in the barbel steed (Hemibarbus labeo) and its involvement in monocyte/macrophage and neutrophil migration.
2020,
Pubmed
Kim,
Subfunctionalization and evolution of liver-expressed antimicrobial peptide 2 (LEAP2) isoform genes in Siberian sturgeon (Acipenser baerii), a primitive chondrostean fish species.
2019,
Pubmed
Krause,
Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver.
2003,
Pubmed
Kumar,
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
2018,
Pubmed
Li,
Molecular characterization of the liver-expressed antimicrobial peptide 2 (LEAP-2) in a teleost fish, Plecoglossus altivelis: antimicrobial activity and molecular mechanism.
2015,
Pubmed
Li,
Molecular characterization and functional analysis of two distinct liver-expressed antimicrobial peptide 2 (LEAP-2) genes in large yellow croaker (Larimichthys crocea).
2014,
Pubmed
Liu,
Characterization and functional analysis of liver-expressed antimicrobial peptide-2 (LEAP-2) from golden pompano Trachinotus ovatus (Linnaeus 1758).
2020,
Pubmed
Liu,
Characterization, evolution and functional analysis of the liver-expressed antimicrobial peptide 2 (LEAP-2) gene in miiuy croaker.
2014,
Pubmed
Liu,
Molecular cloning and expression analysis of the liver-expressed antimicrobial peptide 2 (LEAP-2) gene in grass carp.
2010,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Luo,
A novel LEAP-2 in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) confers protection against bacteria-stimulated inflammatory response.
2020,
Pubmed
Mookherjee,
Antimicrobial host defence peptides: functions and clinical potential.
2020,
Pubmed
Myers,
An ancient haplotype containing antimicrobial peptide gene variants is associated with severe fungal skin disease in Persian cats.
2022,
Pubmed
Patocka,
Antimicrobial Peptides: Amphibian Host Defense Peptides.
2019,
Pubmed
Pazgier,
Structural and functional analysis of the pro-domain of human cathelicidin, LL-37.
2013,
Pubmed
Rollins-Smith,
Antimicrobial peptide defenses against chytridiomycosis, an emerging infectious disease of amphibian populations.
2005,
Pubmed
Thiébaud,
Overexpression of Leap2 impairs Xenopus embryonic development and modulates FGF and activin signals.
2016,
Pubmed
,
Xenbase
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Torrent,
Antimicrobial peptide action on parasites.
2012,
Pubmed
Townes,
The interaction of the antimicrobial peptide cLEAP-2 and the bacterial membrane.
2009,
Pubmed
Yang,
Molecular characterization of LEAP-2 cDNA in common carp (Cyprinus carpio L.) and the differential expression upon a Vibrio anguillarum stimulus; indications for a significant immune role in skin.
2014,
Pubmed
Yu,
Antimicrobial and immunomodulatory activity of beta-defensin from the Chinese spiny frog (Quasipaa spinosa).
2022,
Pubmed
Zhu,
Antimicrobial peptide hepcidin contributes to restoration of the intestinal flora after Aeromonas hydrophila infection in Acrossocheilus fasciatus.
2023,
Pubmed
van Hoek,
Antimicrobial peptides in reptiles.
2014,
Pubmed
