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XB-ART-59632
Methods Mol Biol 2023 Jan 01;2630:145-154. doi: 10.1007/978-1-0716-2982-6_11.
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Determining miRNA Expression Patterns in Xenopus.

Antonaci M , Godden AM , Wheeler GN .


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Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20-25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.

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???displayArticle.link??? Methods Mol Biol
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Species referenced: Xenopus tropicalis Xenopus laevis
GO keywords: embryo development


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