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Cell Rep 2022 Dec 13;4111:111802. doi: 10.1016/j.celrep.2022.111802.
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Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.

Hwang H , Yun S , Arcanjo RB , Divyanshi , Chen S , Mei W , Nowak RA , Kwon T , Yang J .

Asymmetric localization of mRNAs is crucial for cell polarity and cell fate determination. By performing fractionation RNA-seq, we report here that a large number of maternal RNAs are associated with the ER in Xenopus oocytes but are released into the cytosol after oocyte maturation. We provide evidence that the majority of ER-associated RNA-binding proteins (RBPs) remain associated with the ER after oocyte maturation. However, all ER-associated RBPs analyzed exhibit reduced binding to some of their target RNAs after oocyte maturation. Our results further show that the ER is remodeled massively during oocyte maturation, leading to the formation of a widespread tubular ER network in the animal hemisphere that is required for the asymmetric localization of mRNAs in mature eggs. Thus, our findings demonstrate that dynamic regulation of RNA-ER association and remodeling of the ER are important for the asymmetric localization of RNAs during development.

PubMed ID: 36516762
PMC ID: PMC9811979
Article link: Cell Rep
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cldn5 dnd1 esrp1 got1 hspa5 ocm psma1 psma2 psme3 psme4 ptbp1
GO keywords: oocyte maturation [+]
Antibodies: Acta1 Ab6 Elavl Ab 2 Hspa5 Ab3 Tia1 Ab1

GEO Series: GSE199254: NCBI

Article Images: [+] show captions
References [+] :
Aguero, Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex. 2017, Pubmed, Xenbase