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XB-ART-59523
Development 2023 Feb 14; doi: 10.1242/dev.201309.
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Production and characterization of monoclonal antibodies to xenopus proteins.

Horr B , Kurtz R , Pandey A , Hoffstrom BG , Schock E , LaBonne C , Alfandari D .


Abstract
Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identifying subcellular localization and characterizing the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing protocols to produce mouse monoclonal antibodies directed against Xenopus proteins of interest. Here we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple Xenopus proteins and their validation in various experimental approaches. These novel antibodies are now available to the research community through the Developmental Study Hybridoma Bank (DSHB).

PubMed ID: 36786311
Article link: Development


Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: adam13 ascl1 cdh11 dlx3 ets1 eya1 foxd3 fzd7 hnf4a lbh mcrs1 mmp14 myc neurod1 neurog2 pard3 pcdh8l pdgfra pnhd prdm12 rax ror2 rpn1 six1 six3 snai2 sox2 sox3 sox8 sox9 tbxt trpm7 vegt wtip zic1
Antibodies: Adam13 Ab10 Eya1 Ab3 FLAG Ab7 Mmu.Hnf4a Ab1 Pcdh8l Ab3 Rax Ab2 Ror2 Ab5 Snai2 Ab2 Sox8 Ab1 Sox9 Ab3 Vegt Ab3 Wtip Ab1

Phenotypes: Xla Wt + adam33 MO (Fig. 5B)

Article Images: [+] show captions