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The ovipositor cue indole inhibits animal host attraction in Aedes aegypti (Diptera: Culicidae) mosquitoes.
Dekel A
,
Sar-Shalom E
,
Vainer Y
,
Yakir E
,
Bohbot JD
.
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BACKGROUND: Mosquitoes are responsible for disease transmission worldwide. They possess the ability to discriminate between different ecological resources, including nectar sources, animal hosts and oviposition sites, a feature mediated by their olfactory system. Insect repellents, such as N,N-diethyl-meta-toluamide (also called DEET), have been shown to activate and inhibit mosquito odorant receptors, resulting in behavioral modulation. This and other repellents currently available for personal protection against mosquitoes are topically applied to the skin and operate at a short range. In our search for potential long-range inhibitors of attractants to human hosts, we have hypothesized that the shared chemical similarities between indole and DEET may confer the former with the ability to block odorant receptor function and inhibit human host attraction in a similar way as DEET.
METHODS: We used the two-electrode voltage clamp system to assay Xenopus laevis oocytes as a platform to compare the pharmacological effect of commercially available insect repellents and indole on the Aedes aegypti (R)-1-octen-3-ol receptor, OR8, a receptor involved in the decision-making of female mosquitoes to identify human hosts. We also conducted arm-in-a-cage and wind-tunnel bioassays to explore the effect of indole on human host-seeking female Aedes aegypti mosquitoes.
RESULTS: Our results demonstrate that indole inhibited the Aedes aegypti (R)-1-octen-3-ol receptor OR8. In our arm-in-a-cage assay, 1 M of DEET reduced mosquito visits on average by 69.3% while the same indole concentration achieved 97.8% inhibition. This effect of indole on flight visits was dose-dependent and disappeared at 1 μM. In the flight tunnel, indole elicited on average 27.5% lower speed, 42.3% lower upwind velocity and 30.4% higher tortuosity compared to the control.
CONCLUSIONS: Indole significantly inhibits OR8 activation by (R)-1-octen-3-ol, mosquito visits to a human hand and long-range human host-seeking. The volatility of indole may be leveraged to develop a novel insect repellent in the context of personal mosquito protection.
Afify,
Commonly Used Insect Repellents Hide Human Odors from Anopheles Mosquitoes.
2019, Pubmed
Afify,
Commonly Used Insect Repellents Hide Human Odors from Anopheles Mosquitoes.
2019,
Pubmed
Afify,
Insect repellents mediate species-specific olfactory behaviours in mosquitoes.
2020,
Pubmed
Allan,
Evaluation of organic infusions and synthetic compounds mediating oviposition inAedes albopictus andAedes aegypti (Diptera: Culicidae).
1995,
Pubmed
Beehler,
Synergism between chemical attractants and visual cues influencing oviposition of the mosquito,Culex quinquefasciatus (Diptera: Culicidae).
1993,
Pubmed
Bernier,
Analysis of human skin emanations by gas chromatography/mass spectrometry. 2. Identification of volatile compounds that are candidate attractants for the yellow fever mosquito (Aedes aegypti).
2000,
Pubmed
Blackwell,
Electrophysiological investigation of larval water and potential oviposition chemo-attractants for Anopheles gambiae s.s.
2000,
Pubmed
Bohbot,
Insect repellents: modulators of mosquito odorant receptor activity.
2010,
Pubmed
,
Xenbase
Bohbot,
Multiple activities of insect repellents on odorant receptors in mosquitoes.
2011,
Pubmed
,
Xenbase
Bohbot,
Odorant receptor modulation: ternary paradigm for mode of action of insect repellents.
2012,
Pubmed
,
Xenbase
Bohbot,
Molecular characterization of the Aedes aegypti odorant receptor gene family.
2007,
Pubmed
Bohbot,
Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
2009,
Pubmed
,
Xenbase
Chen,
Spore dispersal of fetid Lysurus mokusin by feces of mycophagous insects.
2014,
Pubmed
Collins,
Olfactory cues for oviposition behavior in Toxorhynchites moctezuma and Toxorhynchites amboinensis (Diptera: Culicidae).
2002,
Pubmed
Cork,
Olfactory basis of host location by mosquitoes and other haematophagous Diptera.
1996,
Pubmed
Dekel,
Evolutionarily conserved odorant receptor function questions ecological context of octenol role in mosquitoes.
2016,
Pubmed
Dennis,
Aedes aegypti Mosquitoes Use Their Legs to Sense DEET on Contact.
2019,
Pubmed
Ditzen,
Insect odorant receptors are molecular targets of the insect repellent DEET.
2008,
Pubmed
,
Xenbase
Du,
Electroantennogram and oviposition bioassay responses of Culex quinquefasciatus and Culex tarsalis (Diptera: Culicidae) to chemicals in odors from Bermuda grass infusions.
1999,
Pubmed
Elgaali,
Comparison of long-chain alcohols and other volatile compounds emitted from food-borne and related Gram positive and Gram negative bacteria.
2002,
Pubmed
Gibson,
Visual and olfactory responses of haematophagous Diptera to host stimuli.
1999,
Pubmed
Grant,
Interactions of DEET and Novel Repellents With Mosquito Odorant Receptors.
2020,
Pubmed
,
Xenbase
Hao,
Host-seeking and blood-feeding behavior of Aedes albopictus (Diptera: Culicidae) exposed to vapors of geraniol, citral, citronellal, eugenol, or anisaldehyde.
2008,
Pubmed
Khan,
A study of insect repellents. 1. Effect on the flight and approach by Aedes aegypti.
1972,
Pubmed
Lindh,
Transstadial and horizontal transfer of bacteria within a colony of Anopheles gambiae (Diptera: Culicidae) and oviposition response to bacteria-containing water.
2008,
Pubmed
Lu,
Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae.
2007,
Pubmed
Millar,
Interaction of the Culex quinquefasciatus egg raft pheromone with a natural chemical associated with oviposition sites.
1994,
Pubmed
Millar,
Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusions.
1992,
Pubmed
Pellegrino,
A natural polymorphism alters odour and DEET sensitivity in an insect odorant receptor.
2011,
Pubmed
SMYTH,
Range-finding toxicity data: List VI.
1962,
Pubmed
Sanford,
Gustatory receptor neuron responds to DEET and other insect repellents in the yellow-fever mosquito, Aedes aegypti.
2013,
Pubmed
Schreck,
Spatial action of mosquito repellents.
1970,
Pubmed
Syed,
Mosquitoes smell and avoid the insect repellent DEET.
2008,
Pubmed
Takken,
Odor-mediated behavior of Afrotropical malaria mosquitoes.
1999,
Pubmed
Takken,
Inhibition of host-seeking response and olfactory responsiveness in Anopheles gambiae following blood feeding.
2001,
Pubmed
Turlings,
Isolation and identification of allelochemicals that attract the larval parasitoid,Cotesia marginiventris (Cresson), to the microhabitat of one of its hosts.
1991,
Pubmed
Verhulst,
Attractiveness of volatiles from different body parts to the malaria mosquito Anopheles coluzzii is affected by deodorant compounds.
2016,
Pubmed
Xu,
DEET and other repellents are inhibitors of mosquito odorant receptors for oviposition attractants.
2019,
Pubmed
,
Xenbase
Xu,
Odorant Inhibition in Mosquito Olfaction.
2019,
Pubmed
Zeng,
Methyl dihydrojasmonate and lilial are the constituents with an "off-label" insect repellence in perfumes.
2018,
Pubmed