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Front Cell Dev Biol 2022 Jan 01;10:957211. doi: 10.3389/fcell.2022.957211.
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Nodal asymmetry and hedgehog signaling during vertebrate left-right symmetry breaking.

Negretti MI , Böse N , Petri N , Kremnyov S , Tsikolia N .

Development of visceral left-right asymmetry in bilateria is based on initial symmetry breaking followed by subsequent asymmetric molecular patterning. An important step is the left-sided expression of transcription factor pitx2 which is mediated by asymmetric expression of the nodal morphogen in the left lateral plate mesoderm of vertebrates. Processes leading to emergence of the asymmetric nodal domain differ depending on the mode of symmetry breaking. In Xenopus laevis and mouse embryos, the leftward fluid flow on the ventral surface of the left-right organizer leads through intermediate steps to enhanced activity of the nodal protein on the left side of the organizer and subsequent asymmetric nodal induction in the lateral plate mesoderm. In the chick embryo, asymmetric morphogenesis of axial organs leads to paraxial nodal asymmetry during the late gastrulation stage. Although it was shown that hedgehog signaling is required for initiation of the nodal expression, the mechanism of its asymmetry remains to be clarified. In this study, we established the activation of hedgehog signaling in early chick embryos to further study its role in the initiation of asymmetric nodal expression. Our data reveal that hedgehog signaling is sufficient to induce the nodal expression in competent domains of the chick embryo, while treatment of Xenopus embryos led to moderate nodal inhibition. We discuss the role of symmetry breaking and competence in the initiation of asymmetric gene expression.

PubMed ID: 36172285
PMC ID: PMC9511907
Article link: Front Cell Dev Biol

Species referenced: Xenopus laevis
Genes referenced: nodal nodal1 pitx2 sag shh
GO keywords: Hedgehog signaling complex

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References [+] :
Afzelius, Prevalence and genetics of immotile-cilia syndrome and left-handedness. 2006, Pubmed