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STAR Protoc
2022 Sep 16;33:101622. doi: 10.1016/j.xpro.2022.101622.
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SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos.
Dudley CE
,
van den Goor L
,
Miller AL
.
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Traditional fluorescent proteins exhibit limitations in brightness and photostability that hinder optimal characterization of the dynamic cellular behavior of proteins of interest. SNAP- and Halo-tagging are alternatives to traditional fluorescent protein tagging utilizing bright, stable chemical dyes, which may improve signal-to-noise ratio. However, there has been limited use of this approach in vivo in developing organisms. Here, we present a protocol for implementing SNAP- and Halo-tagging in gastrula-stage Xenopus laevis embryos for live confocal microscopy. For complete details on the use and execution of this protocol, please refer to Varadarajan et al. (2022).
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