XB-ART-59304Dev Growth Differ 2022 Sep 01;647:347-361. doi: 10.1111/dgd.12807.
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The homeodomain transcription factor Ventx2 regulates respiratory progenitor cell number and differentiation timing during Xenopus lung development.
Ventx2 is an Antennapedia superfamily/NK-like subclass homeodomain transcription factor best known for its roles in the regulation of early dorsoventral patterning during Xenopus gastrulation and in the maintenance of neural crest multipotency. In this work we characterize the spatiotemporal expression pattern of ventx2 in progenitor cells of the Xenopus respiratory system epithelium. We find that ventx2 is directly induced by BMP signaling in the ventral foregut prior to nkx2-1, the earliest epithelial marker of the respiratory lineage. Functional studies demonstrate that Ventx2 regulates the number of Nkx2-1/Sox9+ respiratory progenitor cells induced during foregut development, the timing and level of surfactant protein gene expression, and proper tracheal-esophageal separation. Our data suggest that Ventx2 regulates the balance of respiratory progenitor cell expansion and differentiation. While the ventx gene family has been lost from the mouse genome during evolution, humans have retained a ventx2-like gene (VENTX). Finally, we discuss how our findings might suggest a possible function of VENTX in human respiratory progenitor cells.
PubMed ID: 36053777
PMC ID: PMC10088502
Article link: Dev Growth Differ
Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: bmp4 fn1 foxa1 foxa2 nkx2-1 smad1 sox2 sox9 tra ventx2 ventx2.2
GO keywords: lung development
Antibodies: Smad1 Ab12 Sox2 Ab4 Sox9 Ab4 Sox9 Ab5
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