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STAR Protoc
2022 Sep 16;33:101455. doi: 10.1016/j.xpro.2022.101455.
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Protocol for culturing and imaging of ectodermal cells from Xenopus.
Tejeda-Muñoz N
,
Monka J
,
De Robertis EM
.
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The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how this protocol can be applied to visualize lysosomes, macropinocytosis, focal adhesions, Wnt signaling, and cell migration. For complete details on the use and execution of this protocol, please refer to Tejeda-Muñoz et al. (2022).
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