XB-ART-59121Dev Growth Differ 2022 Jun 01;645:254-260. doi: 10.1111/dgd.12790.
Show Gene links Show Anatomy links
ccr7 affects both morphogenesis and differentiation during early Xenopus embryogenesis.
Chemokines play important roles in early embryogenesis, including morphogenesis and cell differentiation, before the immune system is established. We characterized Xenopus laevis CC-type chemokine receptor 7 S (ccr7.S) to clarify its role during early development. ccr7 transcripts were detected ubiquitously in early embryos. Dorsal overexpression of ccr7.S inhibited gastrulation, and ccr7.S mRNA-injected embryos had short axes and widely opened neural folds. Because the Keller sandwich explants of the injected embryos elongated well, ccr7.S might affect cell migration, but not convergent extension movements. Ventral ccr7.S overexpression induced secondary axes and chrd.1 upregulation in gastrula-stage embryos. Animal cap assays showed increased expression of neural and cement gland marker genes at later stages. Ccr7.S knockdown reduced chrd.1 expression and inhibited gastrulation at the dorsal side. Our findings suggest that ccr7.S plays important roles in morphogenetic movement and cell differentiation.
PubMed ID: 35581152
Article link: Dev Growth Differ
Species referenced: Xenopus laevis
Genes referenced: admp ag1 bmp1 bmp4 ccr7 chrd.1 fst hbg1 ncam1 nodal1 nodal3.1 nog odc1 olfml3 pitx1 sia1 szl
GO keywords: cell differentiation
Morpholinos: ccr7 MO1
Article Images: [+] show captions