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Purinergic Signal
2021 Sep 01;173:449-465. doi: 10.1007/s11302-021-09792-9.
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BAC transgenic mice to study the expression of P2X2 and P2Y1 receptors.
Grohmann M
,
Schumacher M
,
Günther J
,
Singheiser SM
,
Nußbaum T
,
Wildner F
,
Gerevich Z
,
Jabs R
,
Hirnet D
,
Lohr C
,
Illes P
,
Schmalzing G
,
Franke H
,
Hausmann R
.
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Extracellular purines are important signaling molecules involved in numerous physiological and pathological processes via the activation of P2 receptors. Information about the spatial and temporal P2 receptor (P2R) expression and its regulation remains crucial for the understanding of the role of P2Rs in health and disease. To identify cells carrying P2X2Rs in situ, we have generated BAC transgenic mice that express the P2X2R subunits as fluorescent fusion protein (P2X2-TagRFP). In addition, we generated a BAC P2Y1R TagRFP reporter mouse expressing a TagRFP reporter for the P2RY1 gene expression. We demonstrate expression of the P2X2R in a subset of DRG neurons, the brain stem, the hippocampus, as well as on Purkinje neurons of the cerebellum. However, the weak fluorescence intensity in our P2X2R-TagRFP mouse precluded tracking of living cells. Our P2Y1R reporter mice confirmed the widespread expression of the P2RY1 gene in the CNS and indicate for the first time P2RY1 gene expression in mouse Purkinje cells, which so far has only been described in rats and humans. Our P2R transgenic models have advanced the understanding of purinergic transmission, but BAC transgenic models appeared not always to be straightforward and permanent reliable. We noticed a loss of fluorescence intensity, which depended on the number of progeny generations. These problems are discussed and may help to provide more successful animal models, even if in future more versatile and adaptable nuclease-mediated genome-editing techniques will be the methods of choice.
Alexander,
Effect of transgene copy number on survival in the G93A SOD1 transgenic mouse model of ALS.
2004, Pubmed
Alexander,
Effect of transgene copy number on survival in the G93A SOD1 transgenic mouse model of ALS.
2004,
Pubmed
Amadio,
P2Y1 receptor switches to neurons from glia in juvenile versus neonatal rat cerebellar cortex.
2007,
Pubmed
Babola,
Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space.
2020,
Pubmed
Becker,
The P2X7 carboxyl tail is a regulatory module of P2X7 receptor channel activity.
2008,
Pubmed
,
Xenbase
Burnstock,
Cellular distribution and functions of P2 receptor subtypes in different systems.
2004,
Pubmed
Burnstock,
Purinergic signalling and disorders of the central nervous system.
2008,
Pubmed
Cockayne,
P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP.
2005,
Pubmed
Cragnolini,
Brain-region specific responses of astrocytes to an in vitro injury and neurotrophins.
2018,
Pubmed
Fallah,
TMEM16A(a)/anoctamin-1 shares a homodimeric architecture with CLC chloride channels.
2011,
Pubmed
,
Xenbase
Fischer,
P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb.
2012,
Pubmed
Franke,
Pathophysiology of astroglial purinergic signalling.
2012,
Pubmed
Franke,
Nucleotide signaling in astrogliosis.
2014,
Pubmed
Franke,
Pathological potential of astroglial purinergic receptors.
2014,
Pubmed
Gong,
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.
2003,
Pubmed
Haeger,
An intramembrane aromatic network determines pentameric assembly of Cys-loop receptors.
2010,
Pubmed
Hausmann,
A hydrophobic residue in position 15 of the rP2X3 receptor slows desensitization and reveals properties beneficial for pharmacological analysis and high-throughput screening.
2014,
Pubmed
Haustein,
Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.
2014,
Pubmed
Heine,
Purines in neurite growth and astroglia activation.
2016,
Pubmed
Heine,
P2Y(1) receptor mediated neuronal fibre outgrowth in organotypic brain slice co-cultures.
2015,
Pubmed
Himmel,
The arginine-rich hexapeptide R4W2 is a stereoselective antagonist at the vanilloid receptor 1: a Ca2+ imaging study in adult rat dorsal root ganglion neurons.
2002,
Pubmed
,
Xenbase
Hollopeter,
Identification of the platelet ADP receptor targeted by antithrombotic drugs.
2001,
Pubmed
,
Xenbase
Illes,
Update of P2X receptor properties and their pharmacology: IUPHAR Review 30.
2021,
Pubmed
Inoue,
Nociceptive signaling of P2X receptors in chronic pain states.
2021,
Pubmed
Jacobson,
Update of P2Y receptor pharmacology: IUPHAR Review 27.
2020,
Pubmed
Jacobson,
Medicinal chemistry of adenosine, P2Y and P2X receptors.
2016,
Pubmed
Jacobson,
Tribute to Prof. Geoffrey Burnstock: transition of purinergicsignaling to drug discovery.
2021,
Pubmed
Jarvis,
The neural-glial purinergic receptor ensemble in chronic pain states.
2010,
Pubmed
Jimenez-Pacheco,
Transient P2X7 Receptor Antagonism Produces Lasting Reductions in Spontaneous Seizures and Gliosis in Experimental Temporal Lobe Epilepsy.
2016,
Pubmed
Kaczmarek-Hajek,
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
2018,
Pubmed
Kaczmarek-Hájek,
Molecular and functional properties of P2X receptors--recent progress and persisting challenges.
2012,
Pubmed
Khakh,
P2X receptors as cell-surface ATP sensors in health and disease.
2006,
Pubmed
Kim,
Development of a Mouse Reporter Strain for the Purinergic P2X2 Receptor.
2020,
Pubmed
Krügel,
Purinergic modulation of extracellular glutamate levels in the nucleus accumbens in vivo.
2004,
Pubmed
Merzlyak,
Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
2007,
Pubmed
Miras-Portugal,
P2 receptor interaction and signalling cascades in neuroprotection.
2019,
Pubmed
Moore,
Regional and cellular distribution of the P2Y(1) purinergic receptor in the human brain: striking neuronal localisation.
2000,
Pubmed
Osoegawa,
Bacterial artificial chromosome libraries for mouse sequencing and functional analysis.
2000,
Pubmed
Richler,
Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.
2011,
Pubmed
Rubini,
Functional P2X7 receptors at cultured hippocampal astrocytes but not neurons.
2014,
Pubmed
Shaner,
Improving the photostability of bright monomeric orange and red fluorescent proteins.
2008,
Pubmed
Simões,
Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation.
2018,
Pubmed
Sperlágh,
P2X7 receptor: an emerging target in central nervous system diseases.
2014,
Pubmed
Stolz,
Homodimeric anoctamin-1, but not homodimeric anoctamin-6, is activated by calcium increases mediated by the P2Y1 and P2X7 receptors.
2015,
Pubmed
,
Xenbase
Surprenant,
Signaling at purinergic P2X receptors.
2009,
Pubmed
Tonazzini,
Co-localization and functional cross-talk between A1 and P2Y1 purine receptors in rat hippocampus.
2007,
Pubmed
Verkhratsky,
Physiology of Astroglia.
2018,
Pubmed
Vintersten,
Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes.
2008,
Pubmed
Vulchanova,
Differential distribution of two ATP-gated channels (P2X receptors) determined by immunocytochemistry.
1996,
Pubmed
Warming,
Simple and highly efficient BAC recombineering using galK selection.
2005,
Pubmed
Xu,
P2X4 Receptor Reporter Mice: Sparse Brain Expression and Feeding-Related Presynaptic Facilitation in the Arcuate Nucleus.
2016,
Pubmed
Yang,
An overview on the generation of BAC transgenic mice for neuroscience research.
2005,
Pubmed
Zhang,
P2Y receptors in neuropathic pain.
2019,
Pubmed