Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Proteome Res
2022 Jun 03;216:1449-1466. doi: 10.1021/acs.jproteome.2c00043.
Show Gene links
Show Anatomy links
Distinct Glycosylation Responses to Spinal Cord Injury in Regenerative and Nonregenerative Models.
Ronan R
,
Kshirsagar A
,
Rebelo AL
,
Sunny A
,
Kilcoyne M
,
Flaherty RO
,
Rudd PM
,
Schlosser G
,
Saldova R
,
Pandit A
,
McMahon SS
.
???displayArticle.abstract???
Traumatic spinal cord injury (SCI) results in disruption of tissue integrity and loss of function. We hypothesize that glycosylation has a role in determining the occurrence of regeneration and that biomaterial treatment can influence this glycosylation response. We investigated the glycosylation response to spinal cord transection in Xenopus laevis and rat. Transected rats received an aligned collagen hydrogel. The response compared regenerative success, regenerative failure, and treatment in an established nonregenerative mammalian system. In a healthy rat spinal cord, ultraperformance liquid chromatography (UPLC) N-glycoprofiling identified complex, hybrid, and oligomannose N-glycans. Following rat SCI, complex and outer-arm fucosylated glycans decreased while oligomannose and hybrid structures increased. Sialic acid was associated with microglia/macrophages following SCI. Treatment with aligned collagen hydrogel had a minimal effect on the glycosylation response. In Xenopus, lectin histochemistry revealed increased levels of N-acetyl-glucosamine (GlcNAc) in premetamorphic animals. The addition of GlcNAc is required for processing complex-type glycans and is a necessary foundation for additional branching. A large increase in sialic acid was observed in nonregenerative animals. This work suggests that glycosylation may influence regenerative success. In particular, loss of complex glycans in rat spinal cord may contribute to regeneration failure. Targeting the glycosylation response may be a promising strategy for future therapies.
Abou-Abbass,
Deciphering glycomics and neuroproteomic alterations in experimental traumatic brain injury: Comparative analysis of aspirin and clopidogrel treatment.
2016, Pubmed
Abou-Abbass,
Deciphering glycomics and neuroproteomic alterations in experimental traumatic brain injury: Comparative analysis of aspirin and clopidogrel treatment.
2016,
Pubmed
Ahuja,
Traumatic Spinal Cord Injury-Repair and Regeneration.
2017,
Pubmed
Akasaka-Manya,
Protective effect of N-glycan bisecting GlcNAc residues on beta-amyloid production in Alzheimer's disease.
2010,
Pubmed
Akasaka-Manya,
Increased bisecting and core-fucosylated N-glycans on mutant human amyloid precursor proteins.
2008,
Pubmed
Akita,
Developmental expression of a unique carbohydrate antigen, Tn antigen, in mouse central nervous tissues.
2001,
Pubmed
Barone,
Developmental patterns and neuropsychological assessment in patients with carbohydrate-deficient glycoconjugate syndrome type IA (phosphomannomutase deficiency).
1999,
Pubmed
Bartus,
Large-scale chondroitin sulfate proteoglycan digestion with chondroitinase gene therapy leads to reduced pathology and modulates macrophage phenotype following spinal cord contusion injury.
2014,
Pubmed
Beattie,
Metamorphosis alters the response to spinal cord transection in Xenopus laevis frogs.
1990,
Pubmed
,
Xenbase
Bradbury,
Chondroitinase ABC promotes functional recovery after spinal cord injury.
2002,
Pubmed
Breen,
Therapeutic Effect of Neurotrophin-3 Treatment in an Injectable Collagen Scaffold Following Rat Spinal Cord Hemisection Injury.
2017,
Pubmed
Chia,
Short O-GalNAc glycans: regulation and role in tumor development and clinical perspectives.
2016,
Pubmed
Chien,
The Modulatory Roles of N-glycans in T-Cell-Mediated Autoimmune Diseases.
2018,
Pubmed
Didangelos,
High-throughput proteomics reveal alarmins as amplifiers of tissue pathology and inflammation after spinal cord injury.
2016,
Pubmed
Ding,
Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury.
2006,
Pubmed
Dumont,
Acute spinal cord injury, part I: pathophysiologic mechanisms.
2001,
Pubmed
Fan,
Linear ordered collagen scaffolds loaded with collagen-binding neurotrophin-3 promote axonal regeneration and partial functional recovery after complete spinal cord transection.
2010,
Pubmed
Fan,
A modified collagen scaffold facilitates endogenous neurogenesis for acute spinal cord injury repair.
2017,
Pubmed
Freeze,
Altered glycan structures: the molecular basis of congenital disorders of glycosylation.
2005,
Pubmed
Gizaw,
Glycoblotting method allows for rapid and efficient glycome profiling of human Alzheimer's disease brain, serum and cerebrospinal fluid towards potential biomarker discovery.
2016,
Pubmed
Gizaw,
A comprehensive glycome profiling of Huntington's disease transgenic mice.
2015,
Pubmed
Green,
Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis.
2007,
Pubmed
Grigorian,
Interleukin-2, Interleukin-7, T cell-mediated autoimmunity, and N-glycosylation.
2012,
Pubmed
Grigorian,
Pathogenesis of multiple sclerosis via environmental and genetic dysregulation of N-glycosylation.
2012,
Pubmed
Haggerty,
Extracellular matrix components as therapeutics for spinal cord injury.
2017,
Pubmed
Ham,
Biomaterial strategies for limiting the impact of secondary events following spinal cord injury.
2018,
Pubmed
Han,
Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats.
2009,
Pubmed
Hirao,
Tn antigen, a marker of potential for metastasis of uterine cervix cancer cells.
1993,
Pubmed
Hoban,
The reduction in immunogenicity of neurotrophin overexpressing stem cells after intra-striatal transplantation by encapsulation in an in situ gelling collagen hydrogel.
2013,
Pubmed
Ip,
Sialoadhesin deficiency ameliorates myelin degeneration and axonopathic changes in the CNS of PLP overexpressing mice.
2007,
Pubmed
Kang,
Proteomic analysis of injured spinal cord tissue proteins using 2-DE and MALDI-TOF MS.
2006,
Pubmed
Kehoe,
FDA approved guidance conduits and wraps for peripheral nerve injury: a review of materials and efficacy.
2012,
Pubmed
Khoury,
Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database.
2011,
Pubmed
Kilcoyne,
Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A.
2019,
Pubmed
Kim,
Biomaterial scaffolds used for the regeneration of spinal cord injury (SCI).
2014,
Pubmed
Kleene,
Glycans and neural cell interactions.
2004,
Pubmed
Kobsar,
Attenuated demyelination in the absence of the macrophage-restricted adhesion molecule sialoadhesin (Siglec-1) in mice heterozygously deficient in P0.
2006,
Pubmed
Kwon,
Pathophysiology and pharmacologic treatment of acute spinal cord injury.
2004,
Pubmed
Lee,
Adhesion molecule expression and regulation on cells of the central nervous system.
1999,
Pubmed
Lee-Liu,
Genome-wide expression profile of the response to spinal cord injury in Xenopus laevis reveals extensive differences between regenerative and non-regenerative stages.
2014,
Pubmed
,
Xenbase
Liu,
Nanofibrous collagen nerve conduits for spinal cord repair.
2012,
Pubmed
Marth,
Mammalian glycosylation in immunity.
2008,
Pubmed
Matus,
Mobility of concanavalin A receptors in myelin and synaptic membranes.
1973,
Pubmed
Morgenstern,
Chondroitin sulphate proteoglycans in the CNS injury response.
2002,
Pubmed
Moriarty,
Encapsulation of primary dopaminergic neurons in a GDNF-loaded collagen hydrogel increases their survival, re-innervation and function after intra-striatal transplantation.
2017,
Pubmed
Norenberg,
The pathology of human spinal cord injury: defining the problems.
2004,
Pubmed
Osimanjiang,
Analysis of N- and O-Linked Glycosylation: Differential Glycosylation after Rat Spinal Cord Injury.
2020,
Pubmed
Palmigiano,
CSF N-glycoproteomics for early diagnosis in Alzheimer's disease.
2016,
Pubmed
Perry,
The blood-brain barrier regulates the expression of a macrophage sialic acid-binding receptor on microglia.
1992,
Pubmed
Popovich,
Cellular inflammatory response after spinal cord injury in Sprague-Dawley and Lewis rats.
1997,
Pubmed
Rabinovich,
Regulatory circuits mediated by lectin-glycan interactions in autoimmunity and cancer.
2012,
Pubmed
Rebelo,
Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.
2020,
Pubmed
Royle,
HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software.
2008,
Pubmed
Ruoslahti,
Brain extracellular matrix.
1996,
Pubmed
Russo,
Neoglucosylated collagen matrices drive neuronal cells to differentiate.
2014,
Pubmed
SIMS,
Transection of the spinal cord in developing Xenopus laevis.
1962,
Pubmed
,
Xenbase
Saldova,
Association of N-glycosylation with breast carcinoma and systemic features using high-resolution quantitative UPLC.
2014,
Pubmed
Saldova,
Increase in sialylation and branching in the mouse serum N-glycome correlates with inflammation and ovarian tumour progression.
2013,
Pubmed
Samal,
Region-Specific Characterization of N-Glycans in the Striatum and Substantia Nigra of an Adult Rodent Brain.
2020,
Pubmed
Snider,
A novel composite type I collagen scaffold with micropatterned porosity regulates the entrance of phagocytes in a severe model of spinal cord injury.
2017,
Pubmed
Stanta,
Identification of N-glycosylation changes in the CSF and serum in patients with schizophrenia.
2010,
Pubmed
Talac,
Animal models of spinal cord injury for evaluation of tissue engineering treatment strategies.
2004,
Pubmed
Taniguchi,
Glycans and cancer: role of N-glycans in cancer biomarker, progression and metastasis, and therapeutics.
2015,
Pubmed
Taylor,
Macrophage receptors and immune recognition.
2005,
Pubmed
Varki,
Biological Roles of Glycans
2009,
Pubmed
Wang,
Glycosylation of microtubule-associated protein tau: an abnormal posttranslational modification in Alzheimer's disease.
1996,
Pubmed
Xiao,
One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients.
2016,
Pubmed
Xiao,
Significant Improvement of Acute Complete Spinal Cord Injury Patients Diagnosed by a Combined Criteria Implanted with NeuroRegen Scaffolds and Mesenchymal Stem Cells.
2018,
Pubmed
Yao,
Improved axonal regeneration of transected spinal cord mediated by multichannel collagen conduits functionalized with neurotrophin-3 gene.
2013,
Pubmed
Yoshii,
Restoration of function after spinal cord transection using a collagen bridge.
2004,
Pubmed
Zhao,
Clinical Study of NeuroRegen Scaffold Combined With Human Mesenchymal Stem Cells for the Repair of Chronic Complete Spinal Cord Injury.
2017,
Pubmed
van Kooyk,
Protein-glycan interactions in the control of innate and adaptive immune responses.
2008,
Pubmed
