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J Cell Sci 2021 Dec 15;13424:. doi: 10.1242/jcs.258864.
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Frizzled3 inhibits Vangl2-Prickle3 association to establish planar cell polarity in the vertebrate neural plate.

Chuykin I , Itoh K , Kim K , Sokol SY .

The orientation of epithelial cells in the plane of the tissue, known as planar cell polarity (PCP), is regulated by interactions of asymmetrically localized PCP protein complexes. In the Xenopus neural plate, Van Gogh-like2 (Vangl2) and Prickle3 (Pk3) proteins form a complex at the anterior cell boundaries, but how this complex is regulated in vivo remains largely unknown. Here, we use proximity biotinylation and crosslinking approaches to show that Vangl2-Pk3 association is inhibited by Frizzled3 (Fz3, also known as Fzd3), a core PCP protein that is specifically expressed in the neuroectoderm and is essential for the establishment of PCP in this tissue. This inhibition required Fz3-dependent Vangl2 phosphorylaton. Consistent with our observations, the complex of Pk3 with nonphosphorylatable Vangl2 did not polarize in the neural plate. These findings provide evidence for in vivo regulation of Vangl2-Pk3 complex formation and localization by a Frizzled receptor.

PubMed ID: 34806749
PMC ID: PMC8729781
Article link: J Cell Sci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dsp fzd3 mapk1 prickle3 vangl2
Morpholinos: fzd3 MO1

Phenotypes: Xla Wt + HA-vangl2{T76>A,T78>A} (Fig.S4.A,B) [+]

Article Images: [+] show captions
References [+] :
Adler, The frizzled/stan pathway and planar cell polarity in the Drosophila wing. 2012, Pubmed