Savage AM , Alberio R , Johnson AD .

BB/ T013575/1 Biotechnology and Biological Sciences Research Council

             regeneration

	Fig. 1. Specification of PGCs in non-rodent mammals and primates display similarities during gastrulation despite differential PGC markers during earlier development. (A) Porcine embryos form an amnion after implantation, while (B) primate embryos (illustrated by cynomolgus monkey) form an amnion before implantation. SOX17/BLIMP1+ cells are observed in the porcine posterior epiblast prior to gastrulation, while similar cells are observed in the amnion of primates. However, during gastrulation in both species, increased numbers of PGCs are observed in the posterior epiblast. At E13 pig and E17 cynomolgus monkey, yellow cells indicate T+; SOX17+; TFAP2C+ PGCs induced in GLCM, purple cells indicate migratory SOX17+; TFAP2C+; BLIMP1+ PGCs. Image created in Biorender.
	Fig. 2. Potential stem cell differentiation hierarchy in bPGC species. In species which specify PGCs via GLCM, different stem cell types (including HSCs) may also be specified simultaneously and therefore directly share ancestry with PGCs. Image created in Biorender.
	Fig. 3. Predicted basal vertebrate migratory route for PGCs and HSCs. In Carnegie stage 10 (∼21 days in human embryos) bPGC embryos, pre-migratory bipotential progenitors are associated with the hindgut; Carnegie stage 12 (∼27 days in human embryos) bPGC embryos display migratory cells expressing both PGC and HSC markers. These cells colonise both the genital ridges and ventral wall of the DA, suggesting they respond stochastically to migratory cues from each tissue. Image created in Biorender.
	Fig. 4. Engraftment into different niches can confer a new identify on stem cells. Studies have shown that stem cells are capable of producing cell types relevant to the somatic tissue onto which they are engrafted, suggesting a plasticity of stem cell nature. Image created in Biorender.

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