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Int J Mol Sci 2021 Aug 28;2217:. doi: 10.3390/ijms22179324.
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Ptk7 Is Dynamically Localized at Neural Crest Cell-Cell Contact Sites and Functions in Contact Inhibition of Locomotion.

Grund A , Till K , Giehl K , Borchers A .

Neural crest (NC) cells are highly migratory cells that contribute to various vertebrate tissues, and whose migratory behaviors resemble cancer cell migration and invasion. Information exchange via dynamic NC cell-cell contact is one mechanism by which the directionality of migrating NC cells is controlled. One transmembrane protein that is most likely involved in this process is protein tyrosine kinase 7 (PTK7), an evolutionary conserved Wnt co-receptor that is expressed in cranial NC cells and several tumor cells. In Xenopus, Ptk7 is required for NC migration. In this study, we show that the Ptk7 protein is dynamically localized at cell-cell contact zones of migrating Xenopus NC cells and required for contact inhibition of locomotion (CIL). Using deletion constructs of Ptk7, we determined that the extracellular immunoglobulin domains of Ptk7 are important for its transient accumulation and that they mediate homophilic binding. Conversely, we found that ectopic expression of Ptk7 in non-NC cells was able to prevent NC cell invasion. However, deletion of the extracellular domains of Ptk7 abolished this effect. Thus, Ptk7 is sufficient at protecting non-NC tissue from NC cell invasion, suggesting a common role of PTK7 in contact inhibition, cell invasion, and tissue integrity.

PubMed ID: 34502237
Article link: Int J Mol Sci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: h2bc21 myc ptk7 sox10 sox17a tfap2a twist1
GO keywords: neural crest cell migration [+]
Antibodies: Actb Ab8 Cav1 Ab2 GFP Ab15 HA Ab11 HA Ab3 Myc Ab12 Myc Ab18 Rho Ab15
Morpholinos: ptk7 MO2 ptk7 MO4

Article Images: [+] show captions
References [+] :
Abercrombie, Contact inhibition and malignancy. 1979, Pubmed