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The cell cycle, a 19th century discovery of cytologists, only achieved a satisfactory biochemical explanation in the last 20 years of the 20th century. This personal retrospective focuses on how biochemical studies of the frog egg helped identify the cyclin-based mitotic oscillator and how this approach quickly merged with genetic studies in yeast to establish the basic mechanism of the eukaryotic cell division cycle. The key feature that made this a cyclic process was regulated protein degradation, mediated by ubiquitin, catalyzed by a massive enzyme machine, called the Anaphase Promoting Complex.
Beach,
Functionally homologous cell cycle control genes in budding and fission yeast.
1982, Pubmed
Beach,
Functionally homologous cell cycle control genes in budding and fission yeast.
1982,
Pubmed
Borisy,
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
1967,
Pubmed
Chang,
Atomic structure of the APC/C and its mechanism of protein ubiquitination.
2015,
Pubmed
Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Fang,
Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1.
1998,
Pubmed
,
Xenbase
Fang,
The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation.
1998,
Pubmed
,
Xenbase
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Gard,
Microtubule assembly in cytoplasmic extracts of Xenopus oocytes and eggs.
1987,
Pubmed
,
Xenbase
Gerhart,
A reinvestigation of the role of the grey crescent in axis formation in xenopus laevis.
1981,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Gurdon,
The cytoplasmic control of nuclear activity in animal development.
1968,
Pubmed
Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase
Hartwell,
Genetic control of the cell-division cycle in yeast. I. Detection of mutants.
1970,
Pubmed
King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lörincz,
Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes.
,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Rao,
Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis.
1970,
Pubmed
Rape,
Autonomous regulation of the anaphase-promoting complex couples mitosis to S-phase entry.
2004,
Pubmed
Rusch,
Synchronization of mitosis by the fusion of the plasmodia of Physarum polycephalum.
1966,
Pubmed
Smith,
The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
1971,
Pubmed
Sudakin,
The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.
1995,
Pubmed
Thuriaux,
Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe.
1978,
Pubmed
Zou,
Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.
1999,
Pubmed
,
Xenbase
