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XB-ART-58251
STAR Protoc 2021 Apr 21;22:100552. doi: 10.1016/j.xpro.2021.100552.
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Using RNA-binding proteins for immunoprecipitation of mRNAs from Xenopus laevis embryos.

Martynova NY , Parshina EA , Zaraisky AG .


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This protocol is developed for identifying mRNAs that form complexes with mRNA-binding proteins (mRBPs) in Xenopus laevis embryos at different developmental stages. Here, we describe the use of the Ybx1 mRBP for immunoprecipitation-based mRNA isolation. This protocol features the translation of the mRBP of interest directly in living embryos following injection of synthetic mRNA templates encoding a hybrid of this protein with a specific tag. This approach allows precipitation of mRNA-protein complexes from embryonic lysates using commercially available anti-tag antibodies. For complete details on the use and execution of this protocol, please refer to Parshina et al. (2020).

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Species referenced: Xenopus laevis

References [+] :
Guo, Nanog RNA-binding proteins YBX1 and ILF3 affect pluripotency of embryonic stem cells. 2016, Pubmed